Literature summary for 5.6.2.3 extracted from

Ivessa, A.S.; Zhou, J.Q.; Schulz, V.P.; Monson, E.K.; Zakian, V.A.
Saccharomyces Rrm3p, a 5' to 3' DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA (2002), Genes Dev., 16, 1383-1396.

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Activating Compound

Activating Compound	Comment	Organism	Structure
single-stranded DNA	stimulates	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
expression of a truncated version of Rrm3p as a GST fusion protein in Saccharomyces cerevisiae. This polypeptide (Rrm3pDELTAN), contains amino acids 194 to 723 of the 723-amino-acid protein, including all seven helicase motifs as well as 56 amino acids amino-terminal of the first helicase motif. Rrm3pDELTAN is expressed under the control of a galactose-inducible promoter	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Saccharomyces cerevisiae	5' to 3' DNA helicase. The ATPase/helicase activity of Rrm3p is required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p is telomere-associated in vivo, it likely has a direct role in telomere replication	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P07271	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Saccharomyces cerevisiae	ADP + phosphate	-	?
ATP + H2O	5' to 3' DNA helicase. The ATPase/helicase activity of Rrm3p is required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p is telomere-associated in vivo, it likely has a direct role in telomere replication	Saccharomyces cerevisiae	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
5' to 3' DNA helicase	-	Saccharomyces cerevisiae
Rrm3p	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)