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Literature summary for 5.6.2.3 extracted from

  • Biswas, E.E.; Fricke W.M.; Chen, P.H.; Biswas, S.B.
    Yeast DNA helicase A: cloning, expression, purification, and enzymatic characterization (1997), Biochemistry, 36, 13277-13284.
    View publication on PubMed
Search for more literature for 5.6.2.3

Activating Compound

Activating Compound Comment Organism Structure
single-stranded DNA stimulates activity Saccharomyces cerevisiae
single-stranded DNA binding protein of Escherichia coli (SSB), stimulates to a lower extent Saccharomyces cerevisiae
yeast replication protein A stimulates significantly Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
NaCl ATPase activity is inhibited by salt (NaCl) above 50 mM with a half-maximal inhibition at about 110 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 ATP pH 7.5, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 6 * 90000, SDS-PAGE Saccharomyces cerevisiae
500000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O hydrolyzes ATP and dATP with equal efficiency. ATPase activity of the enzyme is absolutely DNA-dependent. DNA sequences containing pyrimidine stretches are more effective activators than those containing purine stretches. poly(dC) appears to be the most effective activator of the ATPase activity. DNA helicase migrates on a DNA template in 5' to 3' direction Saccharomyces cerevisiae ADP + phosphate
-
 ?
dATP + H2O hydrolyzes ATP and dATP with equal efficiency. ATPase activity of the enzyme is absolutely DNA-dependent Saccharomyces cerevisiae dADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 90000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
DNA helicase A
-
 Saccharomyces cerevisiae