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Literature summary for 5.6.2.3 extracted from

  • Tanner, J.A.; Watt, R.M.; Chai, Y.B.; Lu, L.Y.; Lin, M.C.; Peiris, J.S.; Poon, L.L.; Kung, H.F.; Huang, J.D.
    The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases (2003), J. Biol. Chem., 278, 39578-39582.
    View publication on PubMed
Search for more literature for 5.6.2.3

Activating Compound

Activating Compound Comment Organism Structure
homopolynucleotides significantly stimulate the ATPase activity (15-25fold) with the exception of poly(G) and poly(dG), which are non-stimulatory. dT24 binds over 10 times more strongly than dA24 Severe acute respiratory syndrome-related coronavirus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 GTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
0.2
-
 dATP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
0.22
-
 dCTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
0.33
-
 ATP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
0.35
-
 dGTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
0.47
-
 CTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
0.55
-
 UTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
0.93
-
 dUTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity is dependent on Severe acute respiratory syndrome-related coronavirus
Mn2+ can substitute for Mg2+, 40% of the efficiency with Mg2+ at 2.5 mM Severe acute respiratory syndrome-related coronavirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Severe acute respiratory syndrome-related coronavirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Severe acute respiratory syndrome-related coronavirus
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus P0C6X7 replicase polyprotein 1ab
-

Purification (Commentary)

Purification (Comment) Organism
-
 Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Severe acute respiratory syndrome-related coronavirus ADP + phosphate
-
 ?
ATP + H2O the enzyme exhibited a preference for ATP, dATP, and dCTP over the other NTP/dNTP substrates Severe acute respiratory syndrome-related coronavirus ADP + phosphate
-
 ?
CTP + H2O
-
 Severe acute respiratory syndrome-related coronavirus CDP + phosphate
-
 ?
dATP + H2O the enzyme exhibited a preference for ATP, dATP, and dCTP over the other NTP/dNTP substrates Severe acute respiratory syndrome-related coronavirus dADP + phosphate
-
 ?
dCTP + H2O the enzyme exhibited a preference for ATP, dATP, and dCTP over the other NTP/dNTP substrates Severe acute respiratory syndrome-related coronavirus dCDP + phosphate
-
 ?
dGTP + H2O
-
 Severe acute respiratory syndrome-related coronavirus dGDP + phosphate
-
 ?
dUTP + H2O
-
 Severe acute respiratory syndrome-related coronavirus dUDP + phosphate
-
 ?
GTP + H2O
-
 Severe acute respiratory syndrome-related coronavirus GDP + phosphate
-
 ?
UTP + H2O
-
 Severe acute respiratory syndrome-related coronavirus UDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Severe acute respiratory syndrome-related coronavirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
 GTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
7.5
-
 dGTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
10.5
-
 UTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
13.9
-
 CTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
14.3
-
 dCTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
14.8
-
 dATP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
14.8
-
 dUTP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus
19.1
-
 ATP pH 6.6, 25°C Severe acute respiratory syndrome-related coronavirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 assay at Severe acute respiratory syndrome-related coronavirus