Application | Comment | Organism |
---|---|---|
nutrition | the enzyme is applicated in Japanese soy sauce fermentation | Stenotrophomonas maltophilia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 14% inhibition at 1 mM | Stenotrophomonas maltophilia | |
CuCl2 | 20% inhibition at 1 mM, 17% at 0.1 mM | Stenotrophomonas maltophilia | |
EDTA | 10% inhibition at 1 mM | Stenotrophomonas maltophilia | |
MgCl2 | 20% inhibition at 1 mM, 12% at 0.1 mM | Stenotrophomonas maltophilia | |
MnCl2 | 22% inhibition at 1 mM, 13% at 0.1 mM | Stenotrophomonas maltophilia | |
ZnCl2 | 7% inhibition at 1 mM | Stenotrophomonas maltophilia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 25% activation at 1 mM | Stenotrophomonas maltophilia | |
additional information | the enzyme is not affected by NiCl2 at 1 mM | Stenotrophomonas maltophilia | |
NaCl | the enzyme is salt-tolerant, over 86% of maximal activity at 16% NaCl, no effect at 1 mM | Stenotrophomonas maltophilia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, SDS-PAGE | Stenotrophomonas maltophilia |
41000 | - |
gel filtration | Stenotrophomonas maltophilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Stenotrophomonas maltophilia | - |
L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Stenotrophomonas maltophilia NYW-81 | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | - |
- |
- |
Stenotrophomonas maltophilia NYW-81 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 250fold from strain NYW-81 to homogeneity by two steps of anion exchange chromatography, hydrophobic interaction and hydroxylapatite chromatography, and gel filtration | Stenotrophomonas maltophilia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain NYW-81, Koji medium, effects of carbon and nitrogen sources for cell growth on the production of enzyme, overview | Stenotrophomonas maltophilia | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
325 | - |
purified enzyme | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-asparagine + H2O | 67% of the activity with L-glutamine | Stenotrophomonas maltophilia | D-aspartate + NH3 | - |
? | |
D-asparagine + H2O | 67% of the activity with L-glutamine | Stenotrophomonas maltophilia NYW-81 | D-aspartate + NH3 | - |
? | |
D-glutamine + H2O | 75% of the activity with L-glutamine | Stenotrophomonas maltophilia | D-glutamate + NH3 | - |
? | |
D-glutamine + H2O | 75% of the activity with L-glutamine | Stenotrophomonas maltophilia NYW-81 | D-glutamate + NH3 | - |
? | |
L-asparagine + H2O | 74% of the activity with L-glutamine | Stenotrophomonas maltophilia | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | 74% of the activity with L-glutamine | Stenotrophomonas maltophilia NYW-81 | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | - |
Stenotrophomonas maltophilia | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | best substrate | Stenotrophomonas maltophilia | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Stenotrophomonas maltophilia NYW-81 | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | best substrate | Stenotrophomonas maltophilia NYW-81 | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Stenotrophomonas maltophilia |
Synonyms | Comment | Organism |
---|---|---|
salt-tolerant glutaminase | - |
Stenotrophomonas maltophilia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Stenotrophomonas maltophilia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, 70% remaining activity, purified enzyme | Stenotrophomonas maltophilia |
70 | - |
10 min, 50% remaining activity, purified enzyme | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Stenotrophomonas maltophilia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | most stable at pH 8.0, 60-80% of maximal activity at pH 5.0-10.0, purified enzyme | Stenotrophomonas maltophilia |