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Literature summary for 3.4.22.B80 extracted from

  • Shin, D.; Mukherjee, R.; Grewe, D.; Bojkova, D.; Baek, K.; Bhattacharya, A.; Schulz, L.; Widera, M.; Mehdipour, A.R.; Tascher, G.; Geurink, P.P.; Wilhelm, A.; van der Heden van Noort, G.J.; Ovaa, H.; Mueller, S.; Knobeloch, K.P.; Rajalingam, K.; Schulman, B.A.; Cinatl, J.; Hummer, G.; Ciesek, S.; Dik, D.i.k.i.
    Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity (2020), Nature, 587, 657-662.
    View publication on PubMed
Search for more literature for 3.4.22.B80

Application

Application Comment Organism
drug development inhibition of SCoV2-PLpro with GRL-0617 impairs the virus-induced cytopathogenic effect, fosters the anti-viral interferon pathway and reduces viral replication in infected cells. These results highlight a dual therapeutic strategy in which targeting of SCoV2-PLpro can suppress SARS-CoV-2 infection and promote anti-viral immunity Severe acute respiratory syndrome coronavirus 2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Severe acute respiratory syndrome coronavirus 2

Inhibitors

Inhibitors Comment Organism Structure
(R)-5-amino-2-methyl-N-(1-(naphthalen-1-yl)ethyl)benzamide i.e. GRL-0617. Inhibition of SCoV2-PLpro with GRL-0617 impairs the virus-induced cytopathogenic effect, fosters the anti-viral interferon pathway and reduces viral replication in infected cells. These results highlight a dual therapeutic strategy in which targeting of SCoV2-PLpro can suppress SARS-CoV-2 infection and promote anti-viral immunity Severe acute respiratory syndrome coronavirus 2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
interferon responsive factor 3 + H2O Severe acute respiratory syndrome coronavirus 2 upon infection, SCoV2-PLpro contributes to the cleavage of ISG15 from interferon responsive factor 3 (IRF3) and attenuates type I interferon responses ?
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 ?

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome coronavirus 2
-
 SARS-CoV-2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Severe acute respiratory syndrome coronavirus 2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
interferon responsive factor 3 + H2O upon infection, SCoV2-PLpro contributes to the cleavage of ISG15 from interferon responsive factor 3 (IRF3) and attenuates type I interferon responses Severe acute respiratory syndrome coronavirus 2 ?
-
 ?
interferon responsive factor 3 + H2O upon infection, SCoV2-PLpro contributes to the cleavage of ISG15 from interferon responsive factor 3 (IRF3) and attenuates type I interferon responses. SCoV2-PLpro preferentially cleaves the ubiquitin-like protein ISG15, whereas SCoV-PLpro predominantly targets ubiquitin chains Severe acute respiratory syndrome coronavirus 2 ?
-
 ?
[ISG15] 7-amido-4-methylcoumarin + H2O the enzyme (SCoV2-PLpro) mediates cleavage of 7-amino-4-methyl coumarin from ISG15-7-amido-4-methyl coumarin better than from [K48-linked di-ubiquitin] 7-amido-4-methylcoumarin Severe acute respiratory syndrome coronavirus 2 ISG15 + 7-amino-4-methylcoumarin
-
 ?
[K48-linked di-ubiquitin] 7-amido-4-methylcoumarin + H2O the enzyme (SCoV2-PLpro) mediates cleavage of 7-amino-4-methyl coumarin from I[ISG15] 7-amido-4-methylcoumarin better than from [K48-linked di-ubiquitin] 7-amido-4-methylcoumarin Severe acute respiratory syndrome coronavirus 2 ?
-
 ?

Synonyms

Synonyms Comment Organism
papain-like protease
-
 Severe acute respiratory syndrome coronavirus 2
PLpro
-
 Severe acute respiratory syndrome coronavirus 2
SARS-CoV-2 PLpro
-
 Severe acute respiratory syndrome coronavirus 2
SCoV2-PLpro
-
 Severe acute respiratory syndrome coronavirus 2

General Information

General Information Comment Organism
physiological function the enzyme regulates SARS-CoV-2 viral spread and innate immunity Severe acute respiratory syndrome coronavirus 2