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Literature summary for 3.4.22.B80 extracted from

  • Park, J.; Ko, J.; Kim, D.; Kim, Y.; Kwon, H.; Jeong, H.; Kim, C.; Park, K.; Lee, W.; Ryu, Y.
    Chalcones isolated from Angelica keiskei inhibit cysteine proteases of SARS-CoV (2016), J. Enzyme Inhib. Med. Chem., 31, 23-30 .
    View publication on PubMed
Search for more literature for 3.4.22.B80

Inhibitors

Inhibitors Comment Organism Structure
(2E)-1-[2,4-dihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, mixed-type inhibition Severe acute respiratory syndrome-related coronavirus
(2E)-1-[2,4-dihydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
(2E)-1-[2-hydroxy-3-(2-hydroxy-3-methylbut-3-en-1-yl)-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
(2E)-1-[2-hydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
(2E)-1-[2-hydroxy-4-methoxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
(2E)-1-[3-(2-hydroperoxy-3-methylbut-3-en-1-yl)-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
(2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG-7-amido-4-methyl coumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
(2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
(2E)-1-[3-[(2E)-6,6-dimethoxy-3-methylhex-2-en-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one substrate: LRLRGG 7-amido-4-methylcoumarin, noncompetitive Severe acute respiratory syndrome-related coronavirus
4,9-dimethoxy-7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, 0.2 mM, 80% inhibition Severe acute respiratory syndrome-related coronavirus
4-methoxy-7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, 0.2 mM, 30% inhibition Severe acute respiratory syndrome-related coronavirus
7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, 0.2 mM, 40% inhibition Severe acute respiratory syndrome-related coronavirus
9-methoxy-7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, 0.2 mM, 30% inhibition Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
 human, i.e. SARS-CoV
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
LRLRGG 7-amido-4-methylcoumarin + H2O
-
 Severe acute respiratory syndrome-related coronavirus LRLRGG 7-amido-4-methylcoumarin
-
 ?
[ISG15] 7-amido-4-methyl coumarin + H2O
-
 Severe acute respiratory syndrome-related coronavirus ?
-
 ?
[ubiquitin] 7-amido-4-methyl coumarin + H2O
-
 Severe acute respiratory syndrome-related coronavirus ?
-
 ?

Synonyms

Synonyms Comment Organism
papain-like protease
-
 Severe acute respiratory syndrome-related coronavirus
PLpro
-
 Severe acute respiratory syndrome-related coronavirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Severe acute respiratory syndrome-related coronavirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Severe acute respiratory syndrome-related coronavirus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0056
-
 7H-furo[3,2-g][1]benzopyran-7-one DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus
0.0121
-
 4,9-dimethoxy-7H-furo[3,2-g][1]benzopyran-7-one DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus
0.013
-
 4,9-dimethoxy-7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus
0.018
-
 7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus
0.0188
-
 4-methoxy-7H-furo[3,2-g][1]benzopyran-7-one DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus
0.0331
-
 9-methoxy-7H-furo[3,2-g][1]benzopyran-7-one DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus
0.0538
-
 9-methoxy-7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus
0.0733
-
 4-methoxy-7H-furo[3,2-g][1]benzopyran-7-one deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0011
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0012
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-(2-hydroperoxy-3-methylbut-3-en-1-yl)-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0026
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-(2-hydroperoxy-3-methylbut-3-en-1-yl)-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0049
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0052
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-(2-hydroperoxy-3-methylbut-3-en-1-yl)-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0056
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2-hydroxy-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0061
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0079
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2,4-dihydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0082
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-3-(2-hydroxy-3-methylbut-3-en-1-yl)-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0092
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-4-methoxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0102
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0102
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0109
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2,4-dihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0113
-
 DeISGylation activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-6,6-dimethoxy-3-methylhex-2-en-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0117
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2,4-dihydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0117
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.013
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2,4-dihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0131
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-3-(2-hydroxy-3-methylbut-3-en-1-yl)-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0154
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-4-methoxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.016
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2,4-dihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0174
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2,4-dihydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0193
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-3-(2-hydroxy-3-methylbut-3-en-1-yl)-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0211
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-6,6-dimethoxy-3-methylhex-2-en-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.026
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-4-methoxy-3-(3-methylbut-2-en-1-yl)phenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0302
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[3-[(2E)-6,6-dimethoxy-3-methylhex-2-en-1-yl]-2,4-dihydroxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0441
-
 deubiquitination activity, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one
0.0464
-
 substrate: LRLRGG 7-amido-4-methylcoumarin, pH 6.8, 37°C Severe acute respiratory syndrome-related coronavirus (2E)-1-[2-hydroxy-3-[(2E)-6-hydroxy-3,7-dimethylocta-2,7-dien-1-yl]-4-methoxyphenyl]-3-(4-hydroxyphenyl)prop-2-en-1-one