BRENDA - Enzyme Database
show all sequences of 3.4.22.B50

The SARS-coronavirus papain-like protease: structure, function and inhibition by designed antiviral compounds

Baez-Santos, Y.M.; St John, S.E.; Mesecar, A.D.; Antiviral Res. 115, 21-38 (2015) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
the enzyme is an attractive antiviral drug target because it is essential for coronaviral replication. Targeting the enzyme with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells
Severe acute respiratory syndrome-related coronavirus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
apoenzyme and enzyme in complexes with ubiquitin, and inhibitors 5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide, N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide, N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide, and N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide, sitting drop vapor diffusion method, apoenzyme by mixing of 1.1-20 mg/ml protein in 20 mM Tris, pH 7.5, 10 mM DTT, with 100 mM sodium citrate, pH 5.2, 3 M ammonium sulfate. Enzyme-inhibitor N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide complex by mixing of 5 mg/ml protein in 20 mM Tris, pH 7.5, 10 mM DTT, with 1 mM inhibitor, 1 M (NH4)2SO4, 50 mM MES, pH 6.5, and 2.5% PEG 400. Enzyme-inhibitor 5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide by mixing of 8 mg/ml protein in 20 mM Tris, pH 7.5, 10 mM DTT with 0.20 mM inhibitor, 1 M LiCl2, 0.1 M MES pH 6.0, 30% PEG 6000. Enzyme-inhibitor N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide or N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide complex by mixing of 6 and 12 mg/ml protein, respectively, in 25 mM Tris, pH 7.5, 100 mM NaCl, 10 mM DTT with 0.20 mM inhibitor, 100 mM sodium citrate, pH 5.5, 40% v/v PEG 600. Enzyme-ubiquitin complex by mixing of 3-12 mg/ml protein and ubiquitin in 20 mM Tris, pH 7.5, with 0.1 M CHES, pH 9.5, 18% PEG 3000, X-ray diffraction structure determination and analysis at 1.4-2.75 A resolution
Severe acute respiratory syndrome-related coronavirus
Engineering
Protein Variants
Commentary
Organism
C112S
site-directed mutagenesis, active site cysteine mutant, forms a noncovalent complex with ubiquitin, crystal structure analysis
Severe acute respiratory syndrome-related coronavirus
Inhibitors
Inhibitors
Commentary
Organism
Structure
(4E)-1,7-bis(3,4-dihydroxyphenyl)hept-4-en-3-one
a natural diarylheptanoide inhibitor
Severe acute respiratory syndrome-related coronavirus
(7R)-5,7-dihydroxy-8-(4-hydroxy-3-methoxyphenyl)-2-(4-hydroxy-4-methylpentyl)-2-methyl-3,4,7,8-tetrahydro-2H,6H-pyrano[3,2-g]chromen-6-one
a natural geranylated flavonoid inhibitor
Severe acute respiratory syndrome-related coronavirus
1,6,6-trimethyl-1,2,6,7,8,9-hexahydrophenanthro[1,2-b]furan-10,11-dione
a natural tanshinone inhibitor
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[(1R)-1-(naphthalen-2-yl)ethyl]benzamide
over 90% inhibition at 0.1 mM
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[(1S)-1-(naphthalen-2-yl)ethyl]benzamide
14% inhibition at 0.1 mM
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[1-(naphthalen-2-yl)ethyl]benzamide
racemat
Severe acute respiratory syndrome-related coronavirus
5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide
R-isomer, a potent, noncovalent, competitive inhibitor
Severe acute respiratory syndrome-related coronavirus
6-Mercaptopurine
-
Severe acute respiratory syndrome-related coronavirus
6-thioguanine
-
Severe acute respiratory syndrome-related coronavirus
Cu2+
30% inhibition at 0.01 mM
Severe acute respiratory syndrome-related coronavirus
hydroxypyridine-2-thione-Zn(II)
-
Severe acute respiratory syndrome-related coronavirus
additional information
enzyme structure, function and inhibition by designed antiviral compounds, overview. No inhibition by NSC158362. Selectivity of naphthalene-based enzyme inhibitors, overview
Severe acute respiratory syndrome-related coronavirus
N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-(4-methoxybenzyl)-1-(naphthalen-1-ylmethyl)piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-ethyl-N-phenyldithiocarbamic acid-Zn(II)
-
Severe acute respiratory syndrome-related coronavirus
N-[(2-methoxypyridin-4-yl)methyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-[3-(acetylamino)benzyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
NSC158011
-
Severe acute respiratory syndrome-related coronavirus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no effect by Mg2+, Ca2+, Mn2+, Co2+, and Ni2+
Severe acute respiratory syndrome-related coronavirus
Zn2+
the fingers domain of the enzyme contains a zinc ion that is tetrahedrally coordinated by four cysteines
Severe acute respiratory syndrome-related coronavirus
Organism
Organism
UniProt
Commentary
Textmining
Severe acute respiratory syndrome-related coronavirus
-
from the Guangdong Province of China
-
Reaction
Reaction
Commentary
Organism
Reaction ID
responsible for the cleavages located at the N-terminus of the replicase polyprotein
catalytic cycle and proposed chemical mechanism of the SARS-coronavirus papain-like protease catalyzed reaction, of the catalytic triad residues Cys112 acts as nucleophile, His273 functions as a general acid-base, and Asp287 is paired with histidine helping to align it and promote deprotonation of Cys112, structure-function relationship, overview
Severe acute respiratory syndrome-related coronavirus
Synonyms
Synonyms
Commentary
Organism
PLpro
-
Severe acute respiratory syndrome-related coronavirus
SARS-coronavirus papain-like protease
-
Severe acute respiratory syndrome-related coronavirus
SARS-CoV papain-like protease
-
Severe acute respiratory syndrome-related coronavirus
SARS-CoV PLpro
-
Severe acute respiratory syndrome-related coronavirus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00049
-
5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.00015
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00032
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00035
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-[(2-methoxypyridin-4-yl)methyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00039
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-[3-(acetylamino)benzyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00049
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.0006
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide
0.0008
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
1,6,6-trimethyl-1,2,6,7,8,9-hexahydrophenanthro[1,2-b]furan-10,11-dione
0.0033
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-ethyl-N-phenyldithiocarbamic acid-Zn(II)
0.0037
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
hydroxypyridine-2-thione-Zn(II)
0.0041
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
(4E)-1,7-bis(3,4-dihydroxyphenyl)hept-4-en-3-one
0.005
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
6-thioguanine
0.005
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
(7R)-5,7-dihydroxy-8-(4-hydroxy-3-methoxyphenyl)-2-(4-hydroxy-4-methylpentyl)-2-methyl-3,4,7,8-tetrahydro-2H,6H-pyrano[3,2-g]chromen-6-one
0.0087
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[(1R)-1-(naphthalen-2-yl)ethyl]benzamide
0.0201
-
racemate, pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[1-(naphthalen-2-yl)ethyl]benzamide
0.0216
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
6-Mercaptopurine
0.059
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(4-methoxybenzyl)-1-(naphthalen-1-ylmethyl)piperidine-4-carboxamide
Application (protein specific)
Application
Commentary
Organism
drug development
the enzyme is an attractive antiviral drug target because it is essential for coronaviral replication. Targeting the enzyme with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells
Severe acute respiratory syndrome-related coronavirus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
apoenzyme and enzyme in complexes with ubiquitin, and inhibitors 5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide, N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide, N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide, and N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide, sitting drop vapor diffusion method, apoenzyme by mixing of 1.1-20 mg/ml protein in 20 mM Tris, pH 7.5, 10 mM DTT, with 100 mM sodium citrate, pH 5.2, 3 M ammonium sulfate. Enzyme-inhibitor N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide complex by mixing of 5 mg/ml protein in 20 mM Tris, pH 7.5, 10 mM DTT, with 1 mM inhibitor, 1 M (NH4)2SO4, 50 mM MES, pH 6.5, and 2.5% PEG 400. Enzyme-inhibitor 5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide by mixing of 8 mg/ml protein in 20 mM Tris, pH 7.5, 10 mM DTT with 0.20 mM inhibitor, 1 M LiCl2, 0.1 M MES pH 6.0, 30% PEG 6000. Enzyme-inhibitor N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide or N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide complex by mixing of 6 and 12 mg/ml protein, respectively, in 25 mM Tris, pH 7.5, 100 mM NaCl, 10 mM DTT with 0.20 mM inhibitor, 100 mM sodium citrate, pH 5.5, 40% v/v PEG 600. Enzyme-ubiquitin complex by mixing of 3-12 mg/ml protein and ubiquitin in 20 mM Tris, pH 7.5, with 0.1 M CHES, pH 9.5, 18% PEG 3000, X-ray diffraction structure determination and analysis at 1.4-2.75 A resolution
Severe acute respiratory syndrome-related coronavirus
Engineering (protein specific)
Protein Variants
Commentary
Organism
C112S
site-directed mutagenesis, active site cysteine mutant, forms a noncovalent complex with ubiquitin, crystal structure analysis
Severe acute respiratory syndrome-related coronavirus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.00015
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00032
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00035
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-[(2-methoxypyridin-4-yl)methyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00039
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-[3-(acetylamino)benzyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.00049
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
0.0006
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide
0.0008
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
1,6,6-trimethyl-1,2,6,7,8,9-hexahydrophenanthro[1,2-b]furan-10,11-dione
0.0033
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-ethyl-N-phenyldithiocarbamic acid-Zn(II)
0.0037
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
hydroxypyridine-2-thione-Zn(II)
0.0041
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
(4E)-1,7-bis(3,4-dihydroxyphenyl)hept-4-en-3-one
0.005
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
6-thioguanine
0.005
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
(7R)-5,7-dihydroxy-8-(4-hydroxy-3-methoxyphenyl)-2-(4-hydroxy-4-methylpentyl)-2-methyl-3,4,7,8-tetrahydro-2H,6H-pyrano[3,2-g]chromen-6-one
0.0087
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[(1R)-1-(naphthalen-2-yl)ethyl]benzamide
0.0201
-
racemate, pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[1-(naphthalen-2-yl)ethyl]benzamide
0.0216
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
6-Mercaptopurine
0.059
-
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
N-(4-methoxybenzyl)-1-(naphthalen-1-ylmethyl)piperidine-4-carboxamide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(4E)-1,7-bis(3,4-dihydroxyphenyl)hept-4-en-3-one
a natural diarylheptanoide inhibitor
Severe acute respiratory syndrome-related coronavirus
(7R)-5,7-dihydroxy-8-(4-hydroxy-3-methoxyphenyl)-2-(4-hydroxy-4-methylpentyl)-2-methyl-3,4,7,8-tetrahydro-2H,6H-pyrano[3,2-g]chromen-6-one
a natural geranylated flavonoid inhibitor
Severe acute respiratory syndrome-related coronavirus
1,6,6-trimethyl-1,2,6,7,8,9-hexahydrophenanthro[1,2-b]furan-10,11-dione
a natural tanshinone inhibitor
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[(1R)-1-(naphthalen-2-yl)ethyl]benzamide
over 90% inhibition at 0.1 mM
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[(1S)-1-(naphthalen-2-yl)ethyl]benzamide
14% inhibition at 0.1 mM
Severe acute respiratory syndrome-related coronavirus
2-methyl-N-[1-(naphthalen-2-yl)ethyl]benzamide
racemat
Severe acute respiratory syndrome-related coronavirus
5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide
R-isomer, a potent, noncovalent, competitive inhibitor
Severe acute respiratory syndrome-related coronavirus
6-Mercaptopurine
-
Severe acute respiratory syndrome-related coronavirus
6-thioguanine
-
Severe acute respiratory syndrome-related coronavirus
Cu2+
30% inhibition at 0.01 mM
Severe acute respiratory syndrome-related coronavirus
hydroxypyridine-2-thione-Zn(II)
-
Severe acute respiratory syndrome-related coronavirus
additional information
enzyme structure, function and inhibition by designed antiviral compounds, overview. No inhibition by NSC158362. Selectivity of naphthalene-based enzyme inhibitors, overview
Severe acute respiratory syndrome-related coronavirus
N-(1,3-benzodioxol-5-ylmethyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-(3-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-(4-fluorobenzyl)-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-(4-methoxybenzyl)-1-(naphthalen-1-ylmethyl)piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-ethyl-N-phenyldithiocarbamic acid-Zn(II)
-
Severe acute respiratory syndrome-related coronavirus
N-[(2-methoxypyridin-4-yl)methyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
N-[3-(acetylamino)benzyl]-1-[(1R)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide
-
Severe acute respiratory syndrome-related coronavirus
NSC158011
-
Severe acute respiratory syndrome-related coronavirus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00049
-
5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide
pH and temperature not specified in the publication
Severe acute respiratory syndrome-related coronavirus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no effect by Mg2+, Ca2+, Mn2+, Co2+, and Ni2+
Severe acute respiratory syndrome-related coronavirus
Zn2+
the fingers domain of the enzyme contains a zinc ion that is tetrahedrally coordinated by four cysteines
Severe acute respiratory syndrome-related coronavirus
General Information
General Information
Commentary
Organism
additional information
enzyme structure and function, active site structure with catalytic triad residues, Cys112, His273 and Asp287 and the oxyanion hole-stabilizing residue Trp107, and catalytic mechanism, detailed overview. The fingers domain of PLpro, which contains a zinc ion that is tetrahedrally coordinated by four cysteines, is essential for catalysis because it maintains the structural integrity of the enzyme
Severe acute respiratory syndrome-related coronavirus
physiological function
the primary function of the enzyme is to process the viral polyprotein in a coordinated manner. An additional function of the enzyme is stripping ubiquitin and ISG15 from host-cell proteins to aid the coronavirus in the evasion of the host innate immune responses, enzyme innate immune functions, overview
Severe acute respiratory syndrome-related coronavirus
General Information (protein specific)
General Information
Commentary
Organism
additional information
enzyme structure and function, active site structure with catalytic triad residues, Cys112, His273 and Asp287 and the oxyanion hole-stabilizing residue Trp107, and catalytic mechanism, detailed overview. The fingers domain of PLpro, which contains a zinc ion that is tetrahedrally coordinated by four cysteines, is essential for catalysis because it maintains the structural integrity of the enzyme
Severe acute respiratory syndrome-related coronavirus
physiological function
the primary function of the enzyme is to process the viral polyprotein in a coordinated manner. An additional function of the enzyme is stripping ubiquitin and ISG15 from host-cell proteins to aid the coronavirus in the evasion of the host innate immune responses, enzyme innate immune functions, overview
Severe acute respiratory syndrome-related coronavirus
Other publictions for EC 3.4.22.B50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752517
Chu
Porcine epidemic diarrhea vir ...
porcine epidemic diarrhea virus
Antiviral Res.
158
199-205
2018
-
-
1
1
1
-
2
4
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
4
-
-
-
-
1
-
2
-
-
1
-
1
1
-
2
2
1
4
-
-
1
-
-
-
-
-
-
-
-
-
3
1
-
-
-
4
-
-
-
-
-
-
-
-
4
4
731149
Baez-Santos
The SARS-coronavirus papain-li ...
Severe acute respiratory syndrome-related coronavirus
Antiviral Res.
115
21-38
2015
-
1
-
1
1
-
20
-
-
2
-
-
-
5
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
16
-
1
-
-
1
1
-
16
20
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
732186
Kong
Structural view and substrate ...
infectious bronchitis virus
J. Biol. Chem.
290
7160-7168
2015
-
-
1
1
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
754545
Mielech
Murine coronavirus ubiquitin- ...
Murine coronavirus
J. Virol.
89
4907-4917
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
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