Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E-64 | - |
Asclepias curassavica | |
NaCl | 1 M, 70% loss of activity | Asclepias curassavica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide | pH 8.5, 42°C, Lineweaver-Burk equation | Asclepias curassavica | |
0.82 | - |
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide | pH 8.5, 42°C, Eddie-Hofstee equation | Asclepias curassavica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23200 | - |
x * 23200, mass spectrometry | Asclepias curassavica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Asclepias curassavica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | - |
Asclepias curassavica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10880 | - |
pH 8.5, 42°C | Asclepias curassavica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Asclepias curassavica | ? | - |
? | |
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide + H2O | - |
Asclepias curassavica | ? | - |
? | |
N-carbobenzoxy-L-Ala 4-nitrophenyl ester + H2O | 47% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester | Asclepias curassavica | N-carbobenzoxy-L-Ala + 4-nitrophenol | - |
? | |
N-carbobenzoxy-L-Gln 4-nitrophenyl ester + H2O | - |
Asclepias curassavica | N-carbobenzoxy-L-Gln + 4-nitrophenol | - |
? | |
N-carbobenzoxy-L-Ile 4-nitrophenyl ester + H2O | 59% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester | Asclepias curassavica | N-carbobenzoxy-L-Ile + 4-nitrophenol | - |
? | |
N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O | 78% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester | Asclepias curassavica | N-carbobenzoxy-L-Lys + 4-nitrophenol | - |
? | |
N-carbobenzoxy-L-Tyr 4-nitrophenyl ester + H2O | 51% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester | Asclepias curassavica | N-carbobenzoxy-L-Tyr + 4-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23200, mass spectrometry | Asclepias curassavica |
Synonyms | Comment | Organism |
---|---|---|
asclepain cI | - |
Asclepias curassavica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
2 h at pH 8.5, stable, 4 h, 30% loss of activity | Asclepias curassavica |
40 | - |
2 h, stable | Asclepias curassavica |
50 | - |
2 h, stable | Asclepias curassavica |
60 | - |
2 h, stable | Asclepias curassavica |
70 | - |
40 min, complete inactivation | Asclepias curassavica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Asclepias curassavica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Asclepias curassavica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
2 h at 37°C, stable, 4 h, 30% loss of activity | Asclepias curassavica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Asclepias curassavica | higher than, isoelectric focusing | - |
9.3 |