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Literature summary for 3.4.22.47 extracted from

  • Abe, N.; Kadowaki, T.; Okamoto, K.; Nakayama, K.; Ohishi, M.; Yamamoto, K.
    Biochemical and functional properties of lysine-specific cysteine proteinase (Lys-gingipain) as a virulence factor of Porphyromonas gingivalis in periodontal disease (1998), J. Biochem., 123, 305-312.
    View publication on PubMed
Search for more literature for 3.4.22.47

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, 79% inhibition Porphyromonas gingivalis
FeCl3 1 mM, 42% inhibition Porphyromonas gingivalis
iodoacetamide 1 mM, 92% inhibition Porphyromonas gingivalis
iodoacetic acid 1 mM, 76% inhibition Porphyromonas gingivalis
leupeptin 0.43 mM, 83% inhibition Porphyromonas gingivalis
MnSO4 1 mM, 50% inhibition Porphyromonas gingivalis
tosyl-L-lysine chloromethyl ketone 1 mM, 96% inhibition Porphyromonas gingivalis
tosyl-L-phenylalanine chloromethyl ketone 1 mM, 99% inhibition Porphyromonas gingivalis
ZnCl2 1 mM, 50% inhibition Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 1 * 51000, SDS-PAGE Porphyromonas gingivalis
52000
-
 gel filtration Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
 gingipain R deficient strain KDP112
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, immunoaffinity column, Mono S Porphyromonas gingivalis

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Porphyromonas gingivalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.356
-
 release of 7-amino-4-methylcoumarin Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
t-butyl-oxycarbonyl-L-Glu-L-Lys-L-Lys-4-methyl-7-coumarylamide + H2O 41% of activity with t-butyl-oxycarbonyl-L-Val-L-Leu-L-Lys-4-methyl-7-coumarylamide Porphyromonas gingivalis t-butyl-oxycarbonyl-L-Glu-L-Lys-L-Lys + 7-amino-4-methylcoumarin
-
 ?
t-butyl-oxycarbonyl-L-Val-L-Leu-L-Lys-4-methyl-7-coumarylamide + H2O gingipain K has a stron preference for substrates containing Lys in the p1 site Porphyromonas gingivalis t-butyl-oxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Porphyromonas gingivalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 no loss of activity after 2 h Porphyromonas gingivalis
60
-
 60% loss of activity after 10 min Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 hydrolysis of t-butyloxycarbonyl-L-Val-L-Leu-L-Lys-4-methyl-7-coumarylamide Porphyromonas gingivalis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 hydrolysis of t-butyl-oxycarbonyl-L-Val-L-Leu-L-Lys-4-methyl-7-coumarylamide Porphyromonas gingivalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 40% loss of activity after 5 min Porphyromonas gingivalis
5.5 10.5 0°C, no loss of activity Porphyromonas gingivalis