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Literature summary for 3.4.22.16 extracted from
- Identification, expression and immunolocalization of cathepsin B3, a stage-specific antigen expressed by juvenile Fasciola gigantica (2009), Acta Trop., 112, 164-173.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | recombinant CatB3 shows enzymatic activity when autoprocessed at pH 4.5 in the presence of dithiothreitol and dextran sulfate | Fasciola gigantica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Fasciola gigantica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27900 | - |
mature enzyme, calculated from amino acid sequence | Fasciola gigantica |
| 34000 | - |
native cathepsin B3, SDS-PAGE | Fasciola gigantica |
| 37000 | - |
pro-CatB3, non-denaturing SDS-PAGE | Fasciola gigantica |
| 38000 | 39000 | deglycosylation of recombinant CatB3 with endoglycosidase H under denaturing and native conditions produces protein migrating at 38000-39000 Da | Fasciola gigantica |
| 39200 | - |
proenzyme, calculated from amino acid sequence | Fasciola gigantica |
| 55000 | 75000 | purified recombinant glycosylated proCatB3 forms migrate at approximately 55000-75000Da | Fasciola gigantica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibronectin + H2O | Fasciola gigantica | - |
? | - |
? |  |
| Gelatin + H2O | Fasciola gigantica | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fasciola gigantica | Q86MW6 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Fasciola gigantica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA column chromatography | Fasciola gigantica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| caecal epithelium | - |
Fasciola gigantica | - |
| larva | cathepsin B3 is detected in the whole body extract of metacercariae and newly excysted juveniles but not in 4-week-old juveniles or adult parasites | Fasciola gigantica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| Fibronectin + H2O | - |
Fasciola gigantica | ? | - |
? | |
| Gelatin + H2O | - |
Fasciola gigantica | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CatB3 | - |
Fasciola gigantica |
| cathepsin B3 | - |
Fasciola gigantica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | CatB3 is a major protease antigen in Fasciola gigantica metacercariae and newly excysted juveniles | Fasciola gigantica |
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