Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | thiol reagents are required for activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CLIK-148 | cathepsin L-specific inhibitor | Homo sapiens | |
CLIK-181 | cathepsin L-specific inhibitor | Homo sapiens | |
E64 | inhibition of gelatinolytic activity | Homo sapiens | |
leupeptin | inhibition of gelatinolytic activity and of autolcleavage of cathepsin L | Homo sapiens | |
additional information | no inhibition by EDTA, PMSF, and pepstatin A | Homo sapiens | |
N-acetyl-Leu-Leumethional | inhibition of gelatinolytic activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the 32 kDa form of cathepsin L is secreted directly from HT-1080 fibrosarcoma cells and not via lysosomal exocytosis, while cathepsin L is secreted via lysosomal exocytosis by several types of cancer cells, including prostate and breast cancer cells. Analysis of the mechanism underlying the secretion of the active 32-kDa cathepsin L form into the medium by HT 1080 cells, overview | Homo sapiens | - |
- |
lysosome | - |
Homo sapiens | 5764 | - |
additional information | cathepsin L is a lysosomal enzyme that is synthesized as a preproform and processed into a 41-kDa proform in the Golgi apparatus. The proform has two fates: to be targeted to lysosomes and to be secreted out of the cells | Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07711 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | investigation of the phosphorylation status of the secreted and lysosomal cathepsin L forms by treatment with alkaline phosphatase (ALP). The 32-kDa form of cathepsin L is phosphorylated, while the 34-kDa form is already dephosphorylated. The 32-kDa form might migrate to the anode faster than the dephosphorylated 34-kDa form because of the retention of the negative charge by the phosphate moiety. Acid phosphatase might remove the phosphorus group from the 32-kDa form as soon as it enters the lysosomes, thereby converting it to the 34-kDa form. The phosphorylated status of 32-kDa cathepsin L suggests that the secreted form has never enters lysosomes | Homo sapiens |
proteolytic modification | cathepsin L is a lysosomal enzyme that is synthesized as a preproform and processed into a 41-kDa proform in the Golgi apparatus. An acidic pH of 4.8 is sufficient to initiate the autoactivation of single-chain cathepsin L intermediates into the mature double-chain form, this process is inhibited by leupeptin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
the enzyme binds to concanavalin A, but not to Lens culinaris agglutinin, Ricinus communis agglutinin 120 (RCA120), and wheat germ agglutinin | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
carcinoma cell | - |
Homo sapiens | - |
fibrosarcoma cell | - |
Homo sapiens | - |
HT-1080 cell | - |
Homo sapiens | - |
prostate gland cancer cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | the enzyme binds to concanavalin A, but not to Lens culinaris agglutinin, Ricinus communis agglutinin 120 (RCA120), and wheat germ agglutinin | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, extracellular form, SDS-PAGE, x * 34000, intracellular form, SDS-PAGE, x * 41000, pro-cathepsin L, SDS-PAGE | Homo sapiens |
More | the active 32-kDa form is not derived from the extracellular processing of 41-kDa procathepsin L by surface activators. With leupeptin treatment, at least five bands are visible in zymography, two 32- and 34-kDa forms with strong activities and three 28.5-, 37-, and 39-kDa forms with faint activities | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cathepsin L | - |
Homo sapiens |
cathepsin L1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
gelatinolytic activity | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | secreted 32 kDA cathepsin is 10times more active than intracellular 34 kDa cathepsin. Membrane trafficking of active 32-kDa cathepsin L, overview | Homo sapiens |