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Literature summary for 3.4.21.68 extracted from
- Potentiating effect of endothelial cells on astrocytic plasminogen activator inhibitor type-1 gene expression in an in vitro model of the blood-brain barrier (2010), Neuroscience, 166, 408-415.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| plasminogen activator inhibitor type-1 | major inhibitor of tissue-type plasminogen activator in the blood | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| plasminogen + H2O | Rattus norvegicus | - |
plasmin + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| astroglial cell | primary astroglial cultures prepared from newborn rat cerebral cortices | Rattus norvegicus | - |
| cell culture | in vitro model of the blood-brain barrier, primary rat astrocyte-enriched cultures are co-cultured with primary adult rat brain microvascular endothelial cells on opposite sides of a transwell membrane | Rattus norvegicus | - |
| endothelial cell | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| plasminogen + H2O | - |
Rattus norvegicus | plasmin + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| t-PA | - |
Rattus norvegicus |
| Tissue-type plasminogen activator | - |
Rattus norvegicus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Rattus norvegicus | influence of endothelial cells on astrocytic gene expression of t-PA using an in vitro model of the blood-brain barrier, primary rat astrocyte-enriched cultures are cocultured with primary adult rat brain microvascular endothelial cells on opposite sides of a transwell membrane, after co-culturing for 9-11 days, the cultures are treated with lipopolysaccharide for 8 h or 24 h, t-PA mRNA expression in astrocytes are unaffected by co-cultivation and/or lipopolysaccharide treatment, analysis of endothelial t-PA gene expression reveals increased unaltered t-PA mRNA levels | additional information |
| Rattus norvegicus | the effects of lipopolysaccharide treatment on the astrocyte levels t-PA transcripts are investigated, lipopolysaccharide does not induce any significant alterations in t-PA gene expression in either monocultured or co-cultured astrocytes | additional information |
| Rattus norvegicus | the influence of astrocytes on endothelial t-PA mRNA expression is studied, for co-cultured endothelial cells, no altered t-PA mRNA levels are observed between the co-cultured cells and the monocultured endothelial cells after treatment with lipopolysaccharide | additional information |
| Rattus norvegicus | when astrocytes are co-cultured with endothelial cells, no significant alterations in the mRNA levels of t-PA mRNA expression are observed in cocultured astrocytes | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | tissue-type plasminogen activator functions as the main activator of the fibrinolytic process in the intravascular compartment | Rattus norvegicus |
| physiological function | tissue-type plasminogen activator is a serine protease that converts plasminogen into the active enzyme plasmin, which in turn degrades the fibrin of the forming thrombus | Rattus norvegicus |
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