Cloned (Comment) | Organism |
---|---|
expression in a baculovirus/insect cells system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C1s(MASP-2 CCP1/2) | chimeric C1s protease containing the MASP-2 complement control protein domain, cleavage efficiency of C4 similar to MASP-2 protease | Homo sapiens |
C1s(MASP-2 SP) | chimeric C1s protease containing the MASP-2 serine protease domain, cleavage efficiency similar to wild-type C1s | Homo sapiens |
D275S/P276I/K405V | similar to wild-type C1s | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
Complement component C4 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C | Homo sapiens | |
0.0052 | - |
Complement component C2 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C | Homo sapiens | |
0.0061 | - |
Complement component C2 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C | Homo sapiens | |
0.0066 | - |
Complement component C4 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
one-step affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C2 + H2O | - |
Homo sapiens | complement component C2a + complement component C2b | - |
? | |
complement component C4 + H2O | - |
Homo sapiens | complement component C4a + complement component C4b | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C1s | - |
Homo sapiens |
complement protease C1s | - |
Homo sapiens |
mannan-binding lectin-associated serine protease-2 | - |
Homo sapiens |
MASP-2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
Complement component C4 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C | Homo sapiens | |
5.1 | - |
Complement component C2 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C | Homo sapiens | |
5.4 | - |
Complement component C4 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C | Homo sapiens | |
5.6 | - |
Complement component C2 | 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C | Homo sapiens |