General Stability | Organism |
---|---|
no inactivation | Vibrio proteolyticus |
urea 8 M, partial inactivation | Vibrio proteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(CH3)2CHCH2CH(NH2)CO-X | X: e.g. succinamide,-CH3,-CH2Cl | Vibrio proteolyticus | |
1,10-phenanthroline | - |
Vibrio proteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
L-Leu-2-naphthylamide | similar value | Vibrio proteolyticus | |
0.51 | - |
L-Leu-L-Val | similar value | Vibrio proteolyticus | |
4.02 | - |
leucinamide | similar value | Vibrio proteolyticus | |
7.2 | - |
L-Leu-Gly | similar value | Vibrio proteolyticus | |
8.5 | - |
L-Leu methyl ester | - |
Vibrio proteolyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Vibrio proteolyticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 4-6 gatom per mol | Vibrio proteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
calculation from sedimentation and diffusion constants | Vibrio proteolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Vibrio proteolyticus | - |
? | - |
? | |
Proteins + H2O | Vibrio proteolyticus | glucagon | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio proteolyticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Vibrio proteolyticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay methods | Vibrio proteolyticus |
Storage Stability | Organism |
---|---|
-20°C, apoenzyme or active enzyme, several years | Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid-peptide + H2O | leucyl-dipeptides | Vibrio proteolyticus | amino acid + peptide | - |
? | |
L-amino acid-peptide + H2O | Leu-Met | Vibrio proteolyticus | amino acid + peptide | - |
? | |
L-amino acid-peptide + H2O | Pro-Phe-Lys | Vibrio proteolyticus | amino acid + peptide | - |
? | |
L-Leu methyl ester | - |
Vibrio proteolyticus | ? | - |
? | |
L-Leu-2-naphthylamide + H2O | - |
Vibrio proteolyticus | L-Leu + 2-naphthylamine | - |
? | |
L-Leu-Gly + H2O | - |
Vibrio proteolyticus | L-Leu + Gly | - |
? | |
L-leucine amide + H2O | - |
Vibrio proteolyticus | L-leucine + NH3 | - |
? | |
Leu-Val + H2O | - |
Vibrio proteolyticus | Leu + Val | - |
? | |
additional information | - |
Vibrio proteolyticus | ? | - |
? | |
additional information | esterase activity | Vibrio proteolyticus | ? | - |
? | |
Proteins + H2O | glucagon | Vibrio proteolyticus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
several h | Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Vibrio proteolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Vibrio proteolyticus |