General Stability | Organism |
---|---|
hexameric LAP is almost completely inactivated, showing 0.6% residual activity, by incubation with 2.5 M GuHCl and 40 mM DTT for 3 h at room temperature | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidinium hydrochloride | hexameric LAP is almost completely inactivated, showing 0.6% residual activity, by incubation with 2.5 M GuHCl and 40 mM DTT for 3 h at room temperature | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.2 | - |
L-leucylamide | native enzyme, pH 8.0, 25°C | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
guanidinium hydrochloride-denatured hexameric leucine aminopeptidase is efficiently refolded by using the cationic detergent cetyltrimethylammonium bromide and the linear polysaccharide dextrin-10 as artificial chaperones leading to 92% of fully active and correct folded LAP, method and kinetics, overview. Recovery of the enzymatic activity using methyl-beta-cyclodextrin was calculated to be 37%, 55%, 64% and 72% after 1 h, 5 h, 10 h and 20 h of incubation, respectively | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Sus scrofa | - |
kidney | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucylamide + H2O | - |
Sus scrofa | L-leucine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | oligomeric structure, overview | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
112 | - |
L-leucylamide | native enzyme, pH 8.0, 25°C | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sus scrofa |