Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 134.4% activity at 1 mM | Aspergillus terreus | |
NaBr | 130.9% activity at 1 mM | Aspergillus terreus | |
NaCl | 120% activity at 1 mM | Aspergillus terreus | |
NH4F | 130.5% activity at 1 mM | Aspergillus terreus | |
Zn(NO3)2 | 155.4% activity at 1 mM | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 87.8% residual activity at 10 mM | Aspergillus terreus | |
Ag+ | 18% residual activity at 10 mM | Aspergillus terreus | |
Al3+ | 20.5% residual activity at 10 mM | Aspergillus terreus | |
Co2+ | 63.5% residual activity at 10 mM | Aspergillus terreus | |
Cu2+ | 28.5% residual activity at 10 mM | Aspergillus terreus | |
EDTA | 47.9% residual activity at 10 mM | Aspergillus terreus | |
Fe2+ | 11.6% residual activity at 10 mM | Aspergillus terreus | |
Fe3+ | 16.5% residual activity at 10 mM | Aspergillus terreus | |
Hg+ | complete inhibition at 10 mM | Aspergillus terreus | |
K+ | 67.1% residual activity at 10 mM | Aspergillus terreus | |
NaBr | 62.7% residual activity at 10 mM | Aspergillus terreus | |
NaH2PO4 | 67.9% residual activity at 10 mM | Aspergillus terreus | |
NH4Cl | 36.6% residual activity at 10 mM | Aspergillus terreus | |
NH4F | 51.3% residual activity at 10 mM | Aspergillus terreus | |
Zn(NO3)2 | 66.2% residual activity at 10 mM | Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.9 | - |
sucrose | in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | |
22.5 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | |
22.7 | - |
sucrose | in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | |
123.6 | - |
cellobiose | in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 136% activity at 1 mM | Aspergillus terreus | |
Ca2+ | 145% activity at 10 mM | Aspergillus terreus | |
Co2+ | 168% activity at 1 mM | Aspergillus terreus | |
Fe3+ | 117% activity at 1 mM | Aspergillus terreus | |
K+ | 124% activity at 1 mM | Aspergillus terreus | |
Mg2+ | 161% activity at 1 mM | Aspergillus terreus | |
Mn2+ | 261% activity at 1 mM | Aspergillus terreus | |
Zn2+ | 126% activity at 1 mM | Aspergillus terreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26900 | - |
1 * 46700 + 1 * 26900, SDS-PAGE | Aspergillus terreus |
46700 | - |
1 * 46700 + 1 * 26900, SDS-PAGE | Aspergillus terreus |
74800 | - |
gel filtration | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography and Sephacryl S-200 gel filtration | Aspergillus terreus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:rye flour-grown cell | - |
Aspergillus terreus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.9 | - |
crude extract, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus |
555 | - |
after 139.4fold purification, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | - |
Aspergillus terreus | ? | - |
? | |
inulin + H2O | worst substrate | Aspergillus terreus | ? | - |
? | |
additional information | no activity with maltose, starch, and trehalose | Aspergillus terreus | ? | - |
? | |
raffinose + H2O | - |
Aspergillus terreus | D-fructose + melibiose | - |
? | |
sucrose + H2O | best substrate | Aspergillus terreus | D-fructose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 46700 + 1 * 26900, SDS-PAGE | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
invertase | - |
Aspergillus terreus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | - |
Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 70 | the enzyme remains stable for 1 h at 55°C when diluted in water. The extracellular invertase is fully stable at lower temperatures, but half-lives of 40 and 5 min are estimated at 60 and 70°C, respectively | Aspergillus terreus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | - |
Aspergillus terreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | the enzyme remains stable for 1 h | Aspergillus terreus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1 | - |
in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | Hg+ |