Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes K181A, D309N, D309N/R429Q and R427Q in Escherichia coli, expression of mutant enzymes in human MG-63 osteosarcoma cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D309N | greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus |
D309N/R427Q | greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus |
K181A | greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus |
R427Q | greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus | PIPKIalpha activity is involved in the actin remodeling that is a prerequisite for efficient phagocytosis. PIPKIalpha appears to contribute to the transient changes in 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate levels that are associated with, and likely required for, the recruitment and regulation of actin-modulating proteins | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hematopoietic cell line | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | PIPKIalpha activity is involved in the actin remodeling that is a prerequisite for efficient phagocytosis. PIPKIalpha appears to contribute to the transient changes in 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate levels that are associated with, and likely required for, the recruitment and regulation of actin-modulating proteins | Mus musculus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |