Cloned (Comment) | Organism |
---|---|
expression of HA-tagged isozyme PI4KIIalpha in HEK-293 cells, co-expression of FLAG-tagged ubiquitin, PI4KIIalpha undergoes multi-ubiquitination | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D308A | enzymatically inactive isozyme PI4KIIalpha | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | - |
Homo sapiens | 5768 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
additional information | Homo sapiens | E3 ubiquitin ligase Itch is a binding partner and regulator of PI4KIIalpha function. Itch directly associates with and ubiquitinates PI4KIIa | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | PI4KIIalpha undergoes multi-ubiquitination via ubiquitin ligase Itch | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
additional information | E3 ubiquitin ligase Itch is a binding partner and regulator of PI4KIIalpha function. Itch directly associates with and ubiquitinates PI4KIIa | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol 4-kinase IIalpha | - |
Homo sapiens |
PI 4-kinase type IIalpha | - |
Homo sapiens |
PI4KIIalpha | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | depletion of PI4KIIalpha by siRNA-mediated knockdown reduces Wnt5a-triggered uptake and/or sorting of Fz4-eGFP into EEA1-positive endosomes. This effect is fully rescued by re-expression of siRNA-resistant PI4KIIalpha | Homo sapiens |
physiological function | key role of isozyme PI4KIIalpha in TGN/endosomal membrane traffic, isozyme PI4KIIalpha regulates Wnt signalling, endosomal sorting of signalling receptors, and promotes adaptor protein recruitment to endosomes and the trans-Golgi network. E3 ubiquitin ligase Itch is a binding partner and regulator of PI4KIIa function. Itch directly associates with and ubiquitinates PI4KIIalpha, and both proteins co-localize on endosomes containing Wnt-activated frizzled 4 receptor. Itch and PI4KIIalpha reciprocally regulate each other | Homo sapiens |