Inhibitors | Comment | Organism | Structure |
---|---|---|---|
F- | 1.5 mM, at pH 8.7 8% inhibition, at pH 6.5 90% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
glucose | 37°C, pH 8.7 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoramidate + D-glucose | Escherichia coli | phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 | D-glucose 1-phosphate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
Crookes strain, ATCC 8739 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.7 | - |
37°C, pH 8.7 | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C or -15°C, 6 years stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoramidate + 2-deoxy-D-glucose | - |
Escherichia coli | 2-deoxy-D-glucose 1-phosphate + NH3 | - |
? | |
phosphoramidate + 3-O-methylglucose | - |
Escherichia coli | 3-O-methylglucose 1-phosphate + NH3 | - |
? | |
phosphoramidate + D-glucose | glucose is best substrate, phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 | Escherichia coli | D-glucose 1-phosphate + NH3 | - |
? | |
phosphoramidate + D-glucose | phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 | Escherichia coli | D-glucose 1-phosphate + NH3 | - |
? | |
phosphoramidate + fructose | - |
Escherichia coli | fructose 1-phosphate + NH3 | - |
? | |
phosphoramidate + glucosamine | - |
Escherichia coli | glucosamine 1-phosphate + NH3 | - |
? | |
phosphoramidate + mannose | - |
Escherichia coli | mannose 1-phosphate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 7.5, more than 30 min, no loss of activity | Escherichia coli |
100 | - |
10 min, about 60% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
assay at | Escherichia coli |