Literature summary for 2.7.1.60 extracted from

North, R.A.; Seizova, S.; Stampfli, A.; Kessans, S.A.; Suzuki, H.; Griffin, M.D.; Kvansakul, M.; Dobson, R.C.
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine kinase from methicillin-resistant Staphylococcus aureus (2014), Acta Crystallogr. Sect. F, 70, 643-649.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M ammonium chloride, 20% (w/v) polyethylene glycol 6000, 0.1 M HEPES pH 7.0	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	x * 35000, SDS-PAGE	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + N-acyl-D-mannosamine	Staphylococcus aureus	-	ADP + N-acyl-D-mannosamine 6-phosphate	-	?
ATP + N-acyl-D-mannosamine	Staphylococcus aureus USA300	-	ADP + N-acyl-D-mannosamine 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus USA300	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + N-acyl-D-mannosamine	-	Staphylococcus aureus	ADP + N-acyl-D-mannosamine 6-phosphate	-	?
ATP + N-acyl-D-mannosamine	-	Staphylococcus aureus USA300	ADP + N-acyl-D-mannosamine 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 35000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
nanK	-	Staphylococcus aureus

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Release 2023.1 (January 2023)