KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ATP | in 100 mM Tris-HCl, pH 7.5, 7 mM MgCl2, 0.5 M KCl, at 42°C | Haloferax volcanii | |
0.31 | - |
D-Fructose 1-phosphate | in 100 mM Tris-HCl, pH 7.5, 7 mM MgCl2, 0.5 M KCl, at 42°C | Haloferax volcanii | |
1.12 | - |
D-fructose 6-phosphate | in 100 mM Tris-HCl, pH 7.5, 7 mM MgCl2, 0.5 M KCl, at 42°C | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the enzyme shows the highest activity at about 0.5M KCl | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Haloferax volcanii |
70000 | - |
gel filtration | Haloferax volcanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | Haloferax volcanii | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | Haloferax volcanii H1209 | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Haloferax volcanii | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | ADP + ? | - |
? | |
ATP + D-fructose 6-phosphate | Haloferax volcanii H1209 | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | ADP + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | D4GYE6 | - |
- |
Haloferax volcanii H1209 | D4GYE6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | Haloferax volcanii | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | Haloferax volcanii H1209 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | Haloferax volcanii | ADP + ? | - |
? | |
ATP + D-fructose 6-phosphate | physiological substrate, the enzyme exhibits a36fold higher catalytic efficiency towards D-fructose 1-phosphate compared to D-fructose 6-phosphate | Haloferax volcanii H1209 | ADP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Haloferax volcanii |
Synonyms | Comment | Organism |
---|---|---|
1-PFK | - |
Haloferax volcanii |
fructose-1-phosphate kinase | - |
Haloferax volcanii |
pfkB | - |
Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Haloferax volcanii |
Organism | Comment | Expression |
---|---|---|
Haloferax volcanii | enzyme expression is specifically upregulated in fructose-grown cells | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in fructose catabolism | Haloferax volcanii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
D-fructose 6-phosphate | in 100 mM Tris-HCl, pH 7.5, 7 mM MgCl2, 0.5 M KCl, at 42°C | Haloferax volcanii | |
1200 | - |
D-Fructose 1-phosphate | in 100 mM Tris-HCl, pH 7.5, 7 mM MgCl2, 0.5 M KCl, at 42°C | Haloferax volcanii |