Cloned (Comment) | Organism |
---|---|
- |
Herpes simplex virus |
Crystallization (Comment) | Organism |
---|---|
sitting and hanging drop vapor diffusion method | Herpes simplex virus |
Protein Variants | Comment | Organism |
---|---|---|
Y101F | 12fold increased Km for thymidine | Herpes simplex virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-exo-methanocarba-thymidine | - |
Herpes simplex virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
thymidine | pH 7.2, wild-type | Herpes simplex virus | |
0.0025 | - |
thymidine | pH 7.2, Y101F mutant | Herpes simplex virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Herpes simplex virus | - |
type 1 | - |
Purification (Comment) | Organism |
---|---|
- |
Herpes simplex virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-exo-methanocarba-thymidine | MCT | Herpes simplex virus | ADP + 2'-exo-methanocarba-thymidine 5'-monophosphate | - |
? | |
ATP + thymidine | - |
Herpes simplex virus | ADP + dTMP | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
2'-exo-methanocarba-thymidine | pH 7.2, wild-type | Herpes simplex virus | |
0.0515 | - |
2'-exo-methanocarba-thymidine | pH 7.2, Y101F mutant | Herpes simplex virus |