General Stability | Organism |
---|---|
dTTP protects against thermal inactivation | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | non-competitive | Tequatrovirus T4 | |
Ba2+ | 0.29 mM | Tequatrovirus T4 | |
dTTP | inhibition dependent on pH | Tequatrovirus T4 | |
Mg2+ | non-competitive | Tequatrovirus T4 | |
Zn2+ | 1.1 mM and 2.9 mM | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially substitute for Mg2+ | Tequatrovirus T4 | |
Co2+ | can partially substitute for Mg2+ | Tequatrovirus T4 | |
Mg2+ | - |
Tequatrovirus T4 | |
Mn2+ | can partially substitute for Mg2+ | Tequatrovirus T4 | |
Ni2+ | can partially substitute for Mg2+ | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Tequatrovirus T4 |
86000 | - |
gel filtration | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Escherichia coli infected with bacteriophage | Tequatrovirus T4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
- |
Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | ATP can be substituted by dATP | Tequatrovirus T4 | ADP + dTMP | - |
? | |
additional information | no phosphorylation of deoxyuridine, 5-halogenated deoxyuridines | Tequatrovirus T4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Tequatrovirus T4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
quick inactivation above, dTTP protects against thermal inactivation | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Tequatrovirus T4 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 60% of maximal activity at pH 5.0, 50% of maximal activity at pH 8.0 | Tequatrovirus T4 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
Mg2+ | pH 7, 37°C | Tequatrovirus T4 | |
3.8 | - |
ATP | pH 7, 37°C | Tequatrovirus T4 |