Inhibitors | Comment | Organism | Structure |
---|---|---|---|
erythro-L-hydroxyaspartate | competitive versus L-aspartate and L-serine | Paracoccus denitrificans | |
L-serine | competitive versus L-aspartate | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
glyoxylate | pH 7.1, 25°C, with substrate L-aspartate | Paracoccus denitrificans | |
1.9 | - |
L-aspartate | pH 7.1, 25°C | Paracoccus denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + L-aspartate | Paracoccus denitrificans | key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism | glycine + oxaloacetate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
inducible | - |
Purification (Comment) | Organism |
---|---|
partial, 40fold | Paracoccus denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.043 | - |
partially purified enzyme | Paracoccus denitrificans |
Storage Stability | Organism |
---|---|
2°C, partially purified enzyme, pH 7.0, quite stable | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + L-aspartate | reverse reaction: specific for glyoxylate as amino acceptor | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
glyoxylate + L-aspartate | poor substrates are asparagine or threo-L-beta-hydroxyaspartate | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
glyoxylate + L-aspartate | favoured reaction, equilibrium in direction of glycine formation | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
glyoxylate + L-aspartate | key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
glyoxylate + L-serine | transamination at about 30% the rate of L-aspartate | Paracoccus denitrificans | hydroxypyruvate + glycine | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | - |
quite stable at pH 7.0 | Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Paracoccus denitrificans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
quite stable at 2°C | Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement | Paracoccus denitrificans |