Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Mesorhizobium loti |
Protein Variants | Comment | Organism |
---|---|---|
M2T/V70K | M2: surface residue | Mesorhizobium loti |
M2T/V70K/E212G | E212: surface residue | Mesorhizobium loti |
M2T/Y35H/V70K | Y35: active site residur | Mesorhizobium loti |
M2T/Y35H/V70K/E212G | E212: surface residue | Mesorhizobium loti |
V70K | active site residue, increase in reactivity toward 2-oxoglutarate | Mesorhizobium loti |
V70K/E212G | E212: surface residue | Mesorhizobium loti |
V70R | active site residue, elimination of activity | Mesorhizobium loti |
V70R/F247C | F247: hydrophobic core residue | Mesorhizobium loti |
Y35H/V70K | active site residues | Mesorhizobium loti |
Y35H/V70R/F247C | F247: hydrophobic core residue | Mesorhizobium loti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.8 | - |
2-oxoglutarate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
12.6 | - |
2-oxoglutarate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
17.2 | - |
2-oxoglutarate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
21.1 | - |
2-oxoglutarate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
29.3 | - |
L-glutamate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
42.4 | - |
L-glutamate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
44.2 | - |
L-glutamate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
51 | - |
L-glutamate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
61.3 | - |
2-oxoglutarate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
77.3 | - |
2-oxoglutarate | M2T/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
80.1 | - |
2-oxoglutarate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
102.6 | - |
2-oxoglutarate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
106.8 | - |
2-oxoglutarate | M2T/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
110.3 | - |
2-oxoglutarate | V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
123.4 | - |
L-glutamate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
133.5 | - |
L-glutamate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
142 | - |
L-glutamate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, SDS-PAGE, gel filtration | Mesorhizobium loti |
154000 | - |
gel filtration | Mesorhizobium loti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | Q988B8 | - |
- |
Purification (Comment) | Organism |
---|---|
hydrophobic interaction chromatography, gel filtration | Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine + 2-oxoglutarate | - |
Mesorhizobium loti | pyridoxal + L-glutamate | - |
r | |
pyridoxamine + pyruvate | - |
Mesorhizobium loti | pyridoxal + L-alanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 41000, SDS-PAGE, gel filtration | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
pyridoxamine-pyruvate aminotransferase | pyridoxamine-2-oxoglutarate aminotransferase activity | Mesorhizobium loti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
L-glutamate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
1.9 | - |
2-oxoglutarate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.7 | - |
L-glutamate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.9 | - |
L-glutamate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
3 | - |
L-glutamate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
3.1 | - |
L-glutamate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
3.5 | - |
2-oxoglutarate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
3.9 | - |
2-oxoglutarate | M2T/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
4 | - |
2-oxoglutarate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
4.1 | - |
2-oxoglutarate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
4.4 | - |
L-glutamate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
4.6 | - |
2-oxoglutarate | M2T/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
4.6 | - |
2-oxoglutarate | V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
5.2 | - |
2-oxoglutarate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
6.4 | - |
L-glutamate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
8.1 | - |
2-oxoglutarate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
9.1 | - |
2-oxoglutarate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
L-glutamate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.019 | - |
L-glutamate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.023 | - |
2-oxoglutarate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.033 | - |
L-glutamate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.04 | - |
2-oxoglutarate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.042 | - |
2-oxoglutarate | V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.043 | - |
2-oxoglutarate | M2T/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.05 | - |
2-oxoglutarate | M2T/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.058 | - |
L-glutamate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.07 | - |
L-glutamate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.1 | - |
L-glutamate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.148 | - |
2-oxoglutarate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.152 | - |
L-glutamate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.167 | - |
2-oxoglutarate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.234 | - |
2-oxoglutarate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.408 | - |
2-oxoglutarate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
0.916 | - |
2-oxoglutarate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti |