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Literature summary for 2.5.1.63 extracted from

  • Ma, L.; Li, Y.; Meng, L.; Deng, H.; Li, Y.; Zhang, Q.; Diao, A.
    Biological fluorination from the sea Discovery of a SAM-dependent nucleophilic fluorinating enzyme from the marine-derived bacterium Streptomyces xinghaiensis NRRL B24674 (2016), RSC Adv., 6, 27047-27051 .
No PubMed abstract available
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Protein Variants

Protein Variants Comment Organism
D16E complete loss of activity, residue is crucial for substrate SAM binding Streptomyces xinghaiensis
F156A complete loss of activity, residue provides the rear of the hydrophobic pocket Streptomyces xinghaiensis
S158A 20% of wild-type activity Streptomyces xinghaiensis
T80A complete loss of activity Streptomyces xinghaiensis
T80S 40% loss of activity Streptomyces xinghaiensis
Y77A almost complete loss of activity Streptomyces xinghaiensis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 25% loss of activity Streptomyces xinghaiensis
Cu2+ about 70% loss of activity Streptomyces xinghaiensis
EDTA about 40% loss of activity Streptomyces xinghaiensis
Fe2+ about 30% loss of activity Streptomyces xinghaiensis
Mg2+ about 40% loss of activity Streptomyces xinghaiensis
Mn2+ about 35% loss of activity Streptomyces xinghaiensis
Ni2+ about 30% loss of activity Streptomyces xinghaiensis
Sn2+ about 30% loss of activity Streptomyces xinghaiensis
Zn2+ about 50% loss of activity Streptomyces xinghaiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis
8.2
-
 fluoride pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis

Organism

Organism UniProt Comment Textmining
Streptomyces xinghaiensis A0A068VNW5
-
-

Storage Stability

Storage Stability Organism
25°C, 50 mM Tris-HCl, pH 7.0, stable for 4 days Streptomyces xinghaiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + chloride
-
 Streptomyces xinghaiensis 5'-deoxy-5'-chloroadenosine + L-methionine
-
 r
S-adenosyl-L-methionine + fluoride
-
 Streptomyces xinghaiensis 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Streptomyces xinghaiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0046
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
 Streptomyces xinghaiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.658
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis