KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics analysis, overview | Helianthus annuus | |
additional information | - |
additional information | kinetics analysis, overview | Hevea brasiliensis | |
additional information | - |
additional information | kinetics analysis, overview | Ficus elastica | |
additional information | - |
additional information | kinetics analysis, overview | Parthenium argentatum | |
additional information | - |
additional information | kinetics analysis, overview | Taraxacum kok-saghyz | |
additional information | - |
additional information | kinetics analysis, overview | Euphorbia lactiflua |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | at the surface of cytoplasmic rubber particles | Helianthus annuus | 5737 | - |
cytoplasm | at the surface of cytoplasmic rubber particles | Hevea brasiliensis | 5737 | - |
cytoplasm | at the surface of cytoplasmic rubber particles | Ficus elastica | 5737 | - |
cytoplasm | at the surface of cytoplasmic rubber particles | Parthenium argentatum | 5737 | - |
cytoplasm | at the surface of cytoplasmic rubber particles | Taraxacum kok-saghyz | 5737 | - |
cytoplasm | at the surface of cytoplasmic rubber particles | Euphorbia lactiflua | 5737 | - |
membrane | bound | Helianthus annuus | 16020 | - |
membrane | bound | Hevea brasiliensis | 16020 | - |
membrane | bound | Ficus elastica | 16020 | - |
membrane | bound | Parthenium argentatum | 16020 | - |
membrane | bound | Taraxacum kok-saghyz | 16020 | - |
membrane | bound | Euphorbia lactiflua | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Helianthus annuus | |
Mg2+ | activates | Hevea brasiliensis | |
Mg2+ | activates | Ficus elastica | |
Mg2+ | activates | Parthenium argentatum | |
Mg2+ | activates | Taraxacum kok-saghyz | |
Mg2+ | activates | Euphorbia lactiflua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Parthenium argentatum | presence of a single rubber transferase capable of using isopentenyl diphosphate in purified rubber particles | ? | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | Helianthus annuus | - |
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | Hevea brasiliensis | - |
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | Ficus elastica | - |
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | Parthenium argentatum | - |
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | Taraxacum kok-saghyz | - |
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | Euphorbia lactiflua | - |
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euphorbia lactiflua | - |
- |
- |
Ficus elastica | - |
- |
- |
Helianthus annuus | - |
- |
- |
Hevea brasiliensis | - |
- |
- |
Parthenium argentatum | - |
- |
- |
Taraxacum kok-saghyz | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Parthenium argentatum makes rubber in parenchyma cells, which must be homogenized to release the rubber particles, the method for purification of enzymatically active rubber particles from the plant requires homogenization that also releases copious amounts of proteases and other degradative enzymes, with a resultant decrease in enzyme stability and half-life, overview | Parthenium argentatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
parenchyma | - |
Parthenium argentatum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | presence of a single rubber transferase capable of using isopentenyl diphosphate in purified rubber particles | Parthenium argentatum | ? | - |
? | |
additional information | assay development for determination of rubber transferase activity in purified rubber particles using photoaffinity-labeled substrate analogues, overview. The molecular weight of the rubber made during in vitro assays is primarily governed by the rate of the chain transfer reaction, that is, the termination and release of an existing rubber polymer molecule, coupled with the initiation of a new molecule in the same active site, the regulation depends strongly upon the identity of substarte and activator, overview | Hevea brasiliensis | ? | - |
? | |
additional information | assay development for determination of rubber transferase activity in purified rubber particles using photoaffinity-labeled substrate analogues, overview. The molecular weight of the rubber made during in vitro assays is primarily governed by the rate of the chain transfer reaction, that is, the termination and release of an existing rubber polymer molecule, coupled with the initiation of a new molecule in the same active site, the regulation depends strongly upon the identity of substarte and activator, overview | Parthenium argentatum | ? | - |
? | |
additional information | assay development for determination of rubber transferase activity in purified rubber particles using photoaffinity-labeled substrate analogues, overview. The molecular weight of the rubber made during in vitro assays is primarily governed by the rate of the chain transfer reaction, that is, the termination and release of an existing rubber polymer molecule, coupled with the initiation of a new molecule in the same active site, the regulation depends strongly upon the identity of substrate and activator, overview | Helianthus annuus | ? | - |
? | |
additional information | assay development for determination of rubber transferase activity in purified rubber particles using photoaffinity-labeled substrate analogues, overview. The molecular weight of the rubber made during in vitro assays is primarily governed by the rate of the chain transfer reaction, that is, the termination and release of an existing rubber polymer molecule, coupled with the initiation of a new molecule in the same active site, the regulation depends strongly upon the identity of substrate and activator, overview | Ficus elastica | ? | - |
? | |
additional information | assay development for determination of rubber transferase activity in purified rubber particles using photoaffinity-labeled substrate analogues, overview. The molecular weight of the rubber made during in vitro assays is primarily governed by the rate of the chain transfer reaction, that is, the termination and release of an existing rubber polymer molecule, coupled with the initiation of a new molecule in the same active site, the regulation depends strongly upon the identity of substrate and activator, overview | Taraxacum kok-saghyz | ? | - |
? | |
additional information | assay development for determination of rubber transferase activity in purified rubber particles using photoaffinity-labeled substrate analogues, overview. The molecular weight of the rubber made during in vitro assays is primarily governed by the rate of the chain transfer reaction, that is, the termination and release of an existing rubber polymer molecule, coupled with the initiation of a new molecule in the same active site, the regulation depends strongly upon the identity of substrate and activator, overview | Euphorbia lactiflua | ? | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | - |
Helianthus annuus | diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | - |
Hevea brasiliensis | diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | - |
Ficus elastica | diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | - |
Parthenium argentatum | diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | - |
Taraxacum kok-saghyz | diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
polycis-polyprenyl diphosphate + isopentenyl diphosphate | - |
Euphorbia lactiflua | diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cis prenyl transferase | - |
Helianthus annuus |
cis prenyl transferase | - |
Hevea brasiliensis |
cis prenyl transferase | - |
Ficus elastica |
cis prenyl transferase | - |
Parthenium argentatum |
cis prenyl transferase | - |
Taraxacum kok-saghyz |
cis prenyl transferase | - |
Euphorbia lactiflua |
rubber transferase | - |
Helianthus annuus |
rubber transferase | - |
Hevea brasiliensis |
rubber transferase | - |
Ficus elastica |
rubber transferase | - |
Parthenium argentatum |
rubber transferase | - |
Taraxacum kok-saghyz |
rubber transferase | - |
Euphorbia lactiflua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | - |
assay at | Parthenium argentatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme activity in rubber particles purified from line 593 decreases below limits of detection in 1 h at 25°C, whereas activity is stable for much longer in particles from line 11591 | Parthenium argentatum |
General Information | Comment | Organism |
---|---|---|
metabolism | the isoprenoid pathway includes the enzymes that can also compete with rubber transferase for isopentenyl pyrophosphate (monomer), allylic pyrophosphate (initiator), and magnesium ions (activator), overview | Helianthus annuus |
metabolism | the isoprenoid pathway includes the enzymes that can also compete with rubber transferase for isopentenyl pyrophosphate (monomer), allylic pyrophosphate (initiator), and magnesium ions (activator), overview | Hevea brasiliensis |
metabolism | the isoprenoid pathway includes the enzymes that can also compete with rubber transferase for isopentenyl pyrophosphate (monomer), allylic pyrophosphate (initiator), and magnesium ions (activator), overview | Ficus elastica |
metabolism | the isoprenoid pathway includes the enzymes that can also compete with rubber transferase for isopentenyl pyrophosphate (monomer), allylic pyrophosphate (initiator), and magnesium ions (activator), overview | Parthenium argentatum |
metabolism | the isoprenoid pathway includes the enzymes that can also compete with rubber transferase for isopentenyl pyrophosphate (monomer), allylic pyrophosphate (initiator), and magnesium ions (activator), overview | Taraxacum kok-saghyz |
metabolism | the isoprenoid pathway includes the enzymes that can also compete with rubber transferase for isopentenyl pyrophosphate (monomer), allylic pyrophosphate (initiator), and magnesium ions (activator), overview | Euphorbia lactiflua |