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Literature summary for 2.5.1.133 extracted from
- The photoheterotrophic growth of bacteriochlorophyll synthase-deficient mutant of Rhodobacter sphaeroides is restored by I44F mutant chlorophyll synthase of Synechocystis sp. PCC 6803 (2016), J. Microbiol. Biotechnol., 26, 959-966 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Cereibacter sphaeroides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F281I | the mutant shows chlorophyll synthase activity. The mutation does not affect the Km value for the original substrate, bacteriochlorophyllide a, but the Km value for the new substrate chlorophyllide a is about 18fold larger than those for the original substrate | Cereibacter sphaeroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
bacteriochlorophyllide a | wild type enzyme, pH and temperature not specified in the publication | Cereibacter sphaeroides |  |
| 0.16 | - |
bacteriochlorophyllide a | mutant enzyme F281I, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate + bacteriochlorophyllide a | Cereibacter sphaeroides | - |
geranylgeranyl-bacteriochlorophyllide a + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | Q9Z5D6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate + bacteriochlorophyllide a | - |
Cereibacter sphaeroides | geranylgeranyl-bacteriochlorophyllide a + diphosphate | - |
? | |
| additional information | no activity with chlorophyllide a | Cereibacter sphaeroides | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacteriochlorophyll synthase | - |
Cereibacter sphaeroides |
| bchG | - |
Cereibacter sphaeroides |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the enzyme-deficient mutant of Rhodobacter sphaeroides, BG1 strain, is photosynthetically incompetent. The function can be restored by I44F mutant chlorophyll synthase of Synechocystis sp. PCC 6803 | Cereibacter sphaeroides |
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