Literature summary for 2.4.2.28 extracted from

Firestone, R.S.; Cameron, S.A.; Karp, J.M.; Arcus, V.L.; Schramm, V.L.
Heat capacity changes for transition-state analogue binding and catalysis with human 5'-methylthioadenosine phosphorylase (2017), ACS Chem. Biol., 12, 464-473 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with 5'-methylthio-DADMe-immucillin A, 5'-butylthio-DADMe-immucillin A, 5'-propylthio-immucillin A or 5'-methylthio-tubercidin, sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M sodium citrate:citric acid (pH 5.5), and 40% (w/v) PEG 400 for 5'-methylthio-DADMe-immucillin A or 5'-propylthio-immucillin A, 3 M sodium chloride and 0.1 M sodium acetate (pH 4.5) for BT-DADMe-immucillin A, and 0.2 M magnesium chloride and 20% (w/v) PEG 3350 for 5'-methylthio-tubercidin	Homo sapiens

Crystallization (Comment)

Organism

in complex with 5'-methylthio-DADMe-immucillin A, 5'-butylthio-DADMe-immucillin A, 5'-propylthio-immucillin A or 5'-methylthio-tubercidin, sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M sodium citrate:citric acid (pH 5.5), and 40% (w/v) PEG 400 for 5'-methylthio-DADMe-immucillin A or 5'-propylthio-immucillin A, 3 M sodium chloride and 0.1 M sodium acetate (pH 4.5) for BT-DADMe-immucillin A, and 0.2 M magnesium chloride and 20% (w/v) PEG 3350 for 5'-methylthio-tubercidin

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
5'-(4-chlorophenyl)thio-DADMe-immucillin A	-	Homo sapiens
5'-butylthio-DADMe-immucillin A	-	Homo sapiens
5'-methylthio-DADMe-immucillin A	-	Homo sapiens
5'-methylthio-immucillin A	-	Homo sapiens
5'-propylthio-DADMe-immucillin A	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-methyl-5'-thioadenosine + phosphate	Homo sapiens	-	adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-methyl-5'-thioadenosine + phosphate	-	Homo sapiens	adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
5'-methylthioadenosine phosphorylase	-	Homo sapiens
MTAP	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.00000001	-	5'-(4-chlorophenyl)thio-DADMe-immucillin A	pH and temperature not specified in the publication	Homo sapiens
0.00000001	-	5'-methylthio-immucillin A	pH and temperature not specified in the publication	Homo sapiens
0.000000086	-	5'-methylthio-DADMe-immucillin A	pH and temperature not specified in the publication	Homo sapiens