Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration, part of the enzyme in supernatant of HepG2 cells | Homo sapiens |
120000 | - |
gel filtration, most of the enzyme in supernatant of HepG2 cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | release of NAMPT enzyme into the cell culture supernatant occurs constitutively. HepG2 lysates and supernatants primarily contain the dimeric form of NAMPT which exhibit similar in vitro specific enzymatic activity | Homo sapiens | - |
hepatocyte | release of NAMPT enzyme into the cell culture supernatant occurs constitutively. In primary hepatocytes, secretion within 24 h is far higher than the cellular content, but is neither influenced by inhibitors of secretion nor by glucose, insulin or TNFalpha. In primary human hepatocytes, secreted NAMPT is less active than intracellular enzyme | Homo sapiens | - |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE, plus some monomer, supernatant of HepG2 cells | Homo sapiens |
monomer | 1 * 55000, SDS-PAGE, plus some dimer, supernatant of HepG2 cells | Homo sapiens |