Literature summary for 2.4.1.B72 extracted from

Niwa, Y.; Nakano, Y.; Suzuki, T.; Yamagishi, M.; Otani, K.; Dohmae, N.; Simizu, S.
Topological analysis of DPY19L3, a human C-mannosyltransferase (2018), FEBS J., 285, 1162-1174 .

Search for more literature for 2.4.1.B72

Cloned(Commentary)

Cloned (Comment)	Organism
transient recombinant expression of wild-type and mutant enzymes in Drosophila melanogaster S2 cells or HEK-293T cells fused to Gaussia luciferase or tagged with myc or myc-His6 tags, quantitative RT-PCR expression analysis	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E206N	site-directed mutatgenesis, the mutation of DPY19L3, resulting in the N-glycosylation consensus sequence (206NFT208), does not induce N-glycosylation, confirming the region is certainly a cytoplasmic region	Homo sapiens
additional information	DPY19L3 has a splice variant, called isoform2, which lacks exons 16-19 and retains part of the intron sequence, resulting in a 542-amino-acid protein. Based on our topological model, isoform2 lacks the C-terminal luminal region of DPY19L3. Topological prediction of isoform2 is almost similar to wild-type DPY19L3, but the last transmembrane (TM) region of wild-type DPY19L3 is changed to re-entrant loop (RL), resulting in the C-terminus of isoform2 is predicted to localize cytoplasm. By redox-sensitive luciferase assay, the C-terminus of isoform2 has high Gaussia luciferase activity, as does this segment of DPY19L3, demonstrating the C-terminus of isoform2 is localized to ER lumen. Furthermore, isoform2 is localized in the ER similar to wild-type DPY19L3	Homo sapiens
N118Q	site-directed mutagenesis of the N-glycosylation site	Homo sapiens
N118Q/N704Q	site-directed mutagenesis of N-glycosylation sites	Homo sapiens
N319Q	site-directed mutagenesis of a potential N-glycosylation site	Homo sapiens
N439Q	site-directed mutagenesis of a potential N-glycosylation site	Homo sapiens
N704Q	site-directed mutagenesis of the N-glycosylation site	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum membrane	a multipass transmembrane protein. DPY19L3 comprises 11 transmembrane regions and two re-entrant loops with the N- and C-terminal ends facing the cytoplasm and ER lumen, respectively. Determination of ER membrane topology of DPY19L3 by redox-sensitive luciferase (Gaussia luciferase, Gluc) assay, overview	Homo sapiens	5789	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q6ZPD9	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	DPY19L3 has four predicted N-glycosylation sites, DPY19L3 is N-glycosylated at Asn118 and Asn704 but not Asn319 and Asn439. N-glycosylations of DPY19L3 do not play any roles for its enzymatic activity	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes from Drosophila melanogaster S2 cells by nickel affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HT-1080 cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
Dpy-19-like protein 3	UniProt	Homo sapiens
DPY19L3	-	Homo sapiens
protein dpy-19 homolog 3	UniProt	Homo sapiens

General Information

General Information	Comment	Organism
additional information	the C-terminal luminal region of DPY19L3 is important for the C-mannosyltransferase activity, while N-glycosylations are not	Homo sapiens
physiological function	enzyme DPY19L3 is a C-mannosyltransferase of R-spondin1 in human cells. DPY19L3 is predicted to be a multipass transmembrane protein that localizes to the endoplasmic reticulum (ER)	Homo sapiens

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Release 2023.1 (January 2023)