Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.B64 extracted from

  • Chen, Y.; Boggess, E.; Ocasio, E.; Warner, A.; Kerns, L.; Drapal, V.; Gossling, C.; Ross, W.; Gourse, R.; Shao, Z.; Dickerson, J.; Mansell, T.; Jarboe, L.
    Reverse engineering of fatty acid-tolerant Escherichia coli identifies design strategies for robust microbial cell factories (2020), Metab. Eng., 61, 120-130 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.B64

Protein Variants

Protein Variants Comment Organism
additional information reverse engineering of a strain of Escherichia coli previously evolved for increased tolerance of octanoic acid (C8), an attractive biorenewable chemical, resulting in increased C8 production, increased butanol tolerance, and altered membrane properties. Evolution is determined to have occurred first through the restoration of WaaG activity, involved in the production of lipopolysaccharides, then an amino acid change in RpoC, a subunit of RNA polymerase, and finally mutation of the BasS-BasR two component system. The WaaG and RpoC mutations both contribute to increased C8 titers, with the RpoC mutation appearing to be the major driver of this effect. Each of these mutations contributes to changes in the cell membrane. Increased membrane integrity and rigidity and decreased abundance of extracellular polymeric substances can be attributed to the restoration of WaaG. The restoration of waaG occurrs first, and relatively quickly, with only the ML115 version of waaG being observed at the end of the second transfer and only the restored version of waaG (waaGR) being observed at the end of the third transfer. The parent strain with restored WaaG (strain YC005) results in a growth rate and final OD. The evolved strain phenotype can be completely attributed to waaGR and rpoCH419P, the basR mutation is not required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P25740
-
-

Synonyms

Synonyms Comment Organism
lipopolysaccharide glucosyltransferase I
-
 Escherichia coli
LPS glucosyltransferase I
-
 Escherichia coli
WaaG
-
 Escherichia coli

General Information

General Information Comment Organism
evolution comparison of the sequence of MG1655, as the reference genome, and of parent strain ML115 reveals the presence of a 768-bp insertion sequence within lipopolysaccharide (LPS) glucosyltransferase I (WaaG). This mutation is implemented unintentionally during the development of ML115. LAR1 and LAR2 both have restored function of WaaG and a single amino acid change within the beta' subunit of RNA polymerase RpoC, and each has a unique mutation in the BasS-BasR two-component signal transduction system. The shared waaG and rpoC mutations are most likely due to the fact that these strains share a common ancestor Escherichia coli
malfunction deletion of waaG has previously been reported to result in a truncated LPS core and loss of flagella. This is consistent with TEM imaging of our strains, in that flagella are visible for LAR1 but not for ML115. Restoration of WaaG increases membrane integrity and increases the membrane rigidity Escherichia coli