Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Biomphalaria glabrata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | 10% (v/v) acetonitrile reduces enzyme activity to about 60% | Biomphalaria glabrata | |
Cu2+ | - |
Biomphalaria glabrata | |
EDTA | no activity in the presence of EDTA | Biomphalaria glabrata | |
Mn2+ | inhibitory at concentrations higher than 80 mM | Biomphalaria glabrata | |
additional information | addition of 5% (v/v) of acetonitrile or the addition of up to 10% (v/v) methanol or glycerol has no negative effect on enzyme activity | Biomphalaria glabrata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
UDP-N-acetyl-alpha-D-galactosamine | with polypeptide Muc2 as cosubstrate, at pH 6.0 and 37°C | Biomphalaria glabrata | |
0.064 | - |
polypeptide Muc2 | at pH 6.0 and 37°C | Biomphalaria glabrata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates activity to a lesser extent than Mn2+ | Biomphalaria glabrata | |
Co2+ | stimulates activity to a lesser extent than Mn2+ | Biomphalaria glabrata | |
Mg2+ | stimulates activity to a lesser extent than Mn2+ | Biomphalaria glabrata | |
Mn2+ | best stimulator of activity | Biomphalaria glabrata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Biomphalaria glabrata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Biomphalaria glabrata | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is O-glycosylated but does not have any putative N-glycosylation sites | Biomphalaria glabrata |
Storage Stability | Organism |
---|---|
-20 to 22°C, 72 h, no loss of activity | Biomphalaria glabrata |
37°C, 72 h, more than 90% loss of activity | Biomphalaria glabrata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a | sequence APPAHGVTSAPDTRPAPGC | Biomphalaria glabrata | 2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a | - |
? | |
2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a' | sequence AHGVTSAPDTR | Biomphalaria glabrata | 2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a' | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc2 | best acceptor polypeptide substrate with sequence PTTTPITTTTTVTPTPTPTGTQTK | Biomphalaria glabrata | UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc2 | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc5Ac | second best acceptor polypeptide with sequence GTTPSPVPTTSTTSAP | Biomphalaria glabrata | UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc5Ac | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Biomphalaria glabrata |
Synonyms | Comment | Organism |
---|---|---|
ppGalNAcT | - |
Biomphalaria glabrata |
UDP-GalNAc:polypeptide GalNAc transferase | - |
Biomphalaria glabrata |
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase | - |
Biomphalaria glabrata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
optimal incubation temperature for short assays up to 2 h | Biomphalaria glabrata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Biomphalaria glabrata |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first step in mucin-type O-glycosylation | Biomphalaria glabrata |