Cloned (Comment) | Organism |
---|---|
full-length cDNA is cloned, a soluble enzyme construct prepared by substituting the honeybee melittin leader sequence for the sequences coding for the cytoplasmic and membrane spanning domains is expressed in insect Sf9 cells using a recombinant baculovirus, nucleotide and amino acid sequence | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
UDP-GalNAc | - |
Bos taurus | |
3.6 | - |
Pro-Pro-Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu-Arg | - |
Bos taurus | |
6.5 | - |
Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Bos taurus | 16020 | - |
membrane | type II membrane protein containing a 23-amino acid transmembrane domain and an 8-amino acid cytoplasmic tail | Bos taurus | 16020 | - |
additional information | intracellular membrane-bound enzyme, localized in the secretory pathway | Bos taurus | - |
- |
soluble | soluble, secreted enzyme is prepared by substituting the honeybee melittin leader sequence for the sequences coding for the cytoplasmic and membrane spanning domains | Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Bos taurus | post-translational, initial reaction in O-linked oligosaccharide biosynthesis | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 3 N- and 1 O-linked oligosaccharide structures per molecule, soluble enzyme contains N-acetylglucosamine, N-acetylgalactosamine, mannose, galactose and fucose, N-linked structures are likely to be of the truncated high-mannose type | Bos taurus |
Purification (Comment) | Organism |
---|---|
21fold, recombinant soluble enzyme, expressed in Sf9 cells | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.16 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly | - |
Bos taurus | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu-Arg | - |
Bos taurus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | bovine apomucin | Bos taurus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptors: synthetic peptides | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | transfer of GalNAc to a serine or threonine residue on the acceptor protein | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | post-translational, initial reaction in O-linked oligosaccharide biosynthesis | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Bos taurus |