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Literature summary for 2.4.1.360 extracted from

  • Nagatomo, Y.; Usui, S.; Ito, T.; Kato, A.; Shimosaka, M.; Taguchi, G.
    Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon (2014), Plant J., 80., 437-448.
    View publication on PubMed
Search for more literature for 2.4.1.360

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) Fagopyrum esculentum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50380
-
 calculated from amino acid sequence Fagopyrum esculentum
50430
-
 calculated from amino acid sequence Fagopyrum esculentum

Organism

Organism UniProt Comment Textmining
Fagopyrum esculentum A0A0A1H7N4
-
-
Fagopyrum esculentum A0A0A1HA03
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fagopyrum esculentum
purification via a nickel-affinity column, concentration using Amicon-Ultra-15 Ultracel-10k Fagopyrum esculentum

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Fagopyrum esculentum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 UDP-alpha-D-glucose + 2-hydroxyeriodictyol
-
 Fagopyrum esculentum 2 UDP + ?
-
 ?
2 UDP-alpha-D-glucose + 2-hydroxynaringenin
-
 Fagopyrum esculentum 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin
-
 ?
2 UDP-alpha-D-glucose + 2-hydroxypinocembrin
-
 Fagopyrum esculentum 2 UDP + ?
-
 ?
2 UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone
-
 Fagopyrum esculentum 2 UDP + ?
-
 ?
2 UDP-alpha-D-glucose + phloretin
-
 Fagopyrum esculentum 2 UDP + ?
-
 ?
additional information no activity with quercetin, luteorin, apigenin Fagopyrum esculentum ?
-
 ?

Synonyms

Synonyms Comment Organism
FcCGT
-
 Fagopyrum esculentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 isozyme CGTa Fagopyrum esculentum
50 55 isozyme CGTb Fagopyrum esculentum

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Fagopyrum esculentum