Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates the enzyme activity | Mycobacterium tuberculosis |
Application | Comment | Organism |
---|---|---|
drug development | WbbL is a good target for the development of antituberculosis therapeutics | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene wbb, overexpression in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Mycobacterium tuberculosis | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | WbbL shows an absolute requirement for divalent cations | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | Mycobacterium tuberculosis | natural acceptor substrate GlcNAc-P-P-decaprenyl | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | Mycobacterium tuberculosis H37Rv | natural acceptor substrate GlcNAc-P-P-decaprenyl | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene wbbL | - |
Mycobacterium tuberculosis H37Rv | - |
gene wbbL | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | kinetic analysis shows that WbbL uses a ternary complex mechanism reaction | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
Mycobacterium tuberculosis | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | natural acceptor substrate GlcNAc-P-P-decaprenyl | Mycobacterium tuberculosis | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
Mycobacterium tuberculosis H37Rv | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | natural acceptor substrate GlcNAc-P-P-decaprenyl | Mycobacterium tuberculosis H37Rv | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cell wall rhamnosyltransferase | - |
Mycobacterium tuberculosis |
WbbL | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | WbbL catalyzes the transfer of an alpha-L-rhamnopyranose residue from dTDP-Rha to decaprenyldiphosphoryl-alpha-D-N-acetyl glucosamine, GlcNAc-P-PDP, to form alpha-L-Rhap-(1->3)-alpha-D-GlcNAc-P-P-DP, which is then further elongated with Galf and Araf units and finally mycolylated and attached to the peptidoglycan. The enzyme is essential for Mycobacterium tuberculosis viability and is absent in eukaryotic cells | Mycobacterium tuberculosis |