Cloned (Comment) | Organism |
---|---|
gene wbbL, expression in and complementation of Mycobacterium smegmatis strain mc2155 temperature-sensitive mutant 2-20/32 | Escherichia coli |
orf Rv3265c or gene wbbL, expression in and complementation of Mycobacterium smegmatis strain mc2155 temperature-sensitive mutant 2-20/32, the enzyme also is capable of restoring an Escherichia coli K12 strain containing an insertionally inactivated wbbL to O-antigen positive, functional expression from plasmid in Escherichia coli strain K12 EMG2 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the Escherichia coli mutant strain K12 EMG2 contains an insertionally inactivated wbbL and is devoid of any O-antigen and shows no WbbL activity, complementation by expression of WbbL from Mycobacterium tuberculosis, a plasmid encoding the putative rhamnosyltransferase gene from Mycobacterium tuberculosis also results in O-antigen synthesis | Escherichia coli |
additional information | the temperature-sensitive mutant contains a single-base change that has a proline changed to a serine residue. Exposure of in mc2155 mutant 2-20/32 to higher non-permissive temperatures results in bacteria that cannot be recovered at the lower permissive temperatures. Complementation of the mutants strain by expression of gene wbbL from Escherichia coli or Mycobacterium tuberculosis | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P36667 | gene wbbL | - |
Escherichia coli WG1 | P36667 | gene wbbL | - |
Mycobacterium tuberculosis | P9WMY3 | orf Rv3265c, gene wbbL | - |
Mycobacterium tuberculosis H37Rv | P9WMY3 | orf Rv3265c, gene wbbL | - |
Mycolicibacterium smegmatis | Q9RN49 | a temperature-sensitive mutant strain 2-20/32, gene wbbL | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | Q9RN49 | a temperature-sensitive mutant strain 2-20/32, gene wbbL | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-rhamnose + GlcNAc-diphospho-trans,octacis-decaprenol | - |
Mycobacterium tuberculosis | ? | - |
? | |
dTDP-beta-L-rhamnose + GlcNAc-diphospho-trans,octacis-decaprenol | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase | - |
Mycobacterium tuberculosis |
dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase | - |
Mycolicibacterium smegmatis |
dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase | - |
Escherichia coli |
WbbL | - |
Mycobacterium tuberculosis |
WbbL | - |
Mycolicibacterium smegmatis |
WbbL | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
37 | - |
assay at | Mycolicibacterium smegmatis |
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycobacterium tuberculosis |
8 | - |
assay at | Mycolicibacterium smegmatis |
8 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | the temperature-sensitive mc2155 strain 2-20/32 mutant shows loss of this transferase activity in 2-20/32 at elevated temperatures, complementation by expression of the enzyme encoded by genes wbbL from Escherichia coli and froms Mycobacterium tuberculosis | Mycolicibacterium smegmatis |
physiological function | the enzyme is required for the rhamnosyl-L-containing linker unit responsible for the attachment of the cell wall polymer mycolyl-arabinogalactan to the peptidoglycan in mycobacteria | Mycobacterium tuberculosis |