Literature summary for 2.4.1.255 extracted from

Liu, L.; Li, L.; Ma, C.; Shi, Y.; Liu, C.; Xiao, Z.; Zhang, Y.; Tian, F.; Gao, Y.; Zhang, J.; Ying, W.; Wang, P.G.; Zhang, L.
O-GlcNAcylation of Thr12/Ser56 in short-form O-GlcNAc transferase (sOGT) regulates its substrate selectivity (2019), J. Biol. Chem., 294, 16620-16633 .

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Protein Variants

Protein Variants	Comment	Organism
S18A/H127A	OGlcNAcylation on the mutant enzyme sharply declines	Homo sapiens
S52A	O-GlcNAcylation on the mutant enzyme increases	Homo sapiens
S56A	O-GlcNAcylation on the mutant enzyme increases	Homo sapiens
T12A	O-GlcNAcylation on the mutant enzyme declines	Homo sapiens
T12A/H127A	OGlcNAcylation on the mutant enzyme sharply declines	Homo sapiens
T12G	sharply reduced O-GlcNAcylation of short-form O-GlcNAc transferase	Homo sapiens
T12L	sharply reduced O-GlcNAcylation of short-form O-GlcNAc transferase	Homo sapiens
T12Y	sharply reduced O-GlcNAcylation of short-form O-GlcNAc transferase	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1757	-	UDP-N-acetyl-alpha-D-glucosamine	37°C, pH not specified in the publication, enzyme form without O-GlcNAcylation	Homo sapiens
0.1805	-	UDP-N-acetyl-alpha-D-glucosamine	37°C, pH not specified in the publication, O-GlcNAcylated enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O15294	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	O-GlcNAcylation of Ser389 in nucleocytoplasmic O-GlcNAc transferase affects its nuclear translocation in HeLa cells. Six O-GlcNAc sites are identified in the tetratricopeptide repeat domain in short-form of O-GlcNAc transferase, with Thr12 and Ser56 being two key sites. Thr12 is a dominant O-GlcNAcylation site. The modification of Ser56 plays a role in regulating short-form of O-GlcNAc transferase. O-GlcNAcylation does not affect activity of O-GlcNAc transferase but alters its substrate selectivity	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293T cell	-	Homo sapiens	-
HeLa cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + FITC-YAVVPVSK peptide	-	Homo sapiens	UDP + ?	-	?

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Release 2023.1 (January 2023)