Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
Y418A | the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch | Corynebacterium glutamicum |
Y418D | the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch | Corynebacterium glutamicum |
Y418F | the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 29-33 from pea starch | Corynebacterium glutamicum |
Y418R | the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch | Corynebacterium glutamicum |
Y418S | the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch | Corynebacterium glutamicum |
Y418W | the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.7 | - |
maltotriose | mutant enzyme Y418A, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
16.3 | - |
maltotriose | mutant enzyme Y418D, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
17.2 | - |
maltotriose | wild type enzyme, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
18 | - |
maltotriose | mutant enzyme Y418S, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
18.2 | - |
maltotriose | mutant enzyme Y418F, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
19.5 | - |
maltotriose | mutant enzyme Y418R, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
20.4 | - |
maltotriose | mutant enzyme Y418W, at pH 6.0 and 30°C | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + glycosyl acceptor | worst substrate | Corynebacterium glutamicum | maltose + D-glucose + maltooligosaccharides | - |
? | |
maltohexaose + glycosyl acceptor | - |
Corynebacterium glutamicum | maltose + D-glucose + maltooligosaccharides | - |
? | |
maltopentaose + glycosyl acceptor | - |
Corynebacterium glutamicum | maltose + D-glucose + maltooligosaccharides | - |
? | |
maltotetraose + glycosyl acceptor | second best substrate | Corynebacterium glutamicum | maltose + D-glucose + maltooligosaccharides | - |
? | |
maltotriose + glycosyl acceptor | best substrate | Corynebacterium glutamicum | maltose + D-glucose + maltooligosaccharides | - |
? | |
pea starch + glycosyl acceptor | - |
Corynebacterium glutamicum | large ring-cyclodextrins 29-33 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
amylomaltase | - |
Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
258 | - |
maltotriose | mutant enzyme Y418R, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
403 | - |
maltotriose | mutant enzyme Y418W, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
1833 | - |
maltotriose | mutant enzyme Y418S, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
1867 | - |
maltotriose | mutant enzyme Y418D, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
1967 | - |
maltotriose | mutant enzyme Y418A, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
2667 | - |
maltotriose | mutant enzyme Y418F, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
2800 | - |
maltotriose | wild type enzyme, at pH 6.0 and 30°C | Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.3 | - |
maltotriose | mutant enzyme Y418R, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
19.7 | - |
maltotriose | mutant enzyme Y418W, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
101.8 | - |
maltotriose | mutant enzyme Y418S, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
115 | - |
maltotriose | mutant enzyme Y418D, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
125.3 | - |
maltotriose | mutant enzyme Y418A, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
146.7 | - |
maltotriose | mutant enzyme Y418F, at pH 6.0 and 30°C | Corynebacterium glutamicum | |
163.3 | - |
maltotriose | wild type enzyme, at pH 6.0 and 30°C | Corynebacterium glutamicum |