Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 14 mM, stimulates | Euonymus alatus | |
EDTA | 1 mM: slight activation, 10 mM: 36% inhibition | Euonymus alatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 35% inhibition | Euonymus alatus | |
Co2+ | 1 mM, 32% inhibition | Euonymus alatus | |
Cu2+ | 1 mM, 94% inhibition | Euonymus alatus | |
dithioerythritol | 10 mM, 13% inhibition | Euonymus alatus | |
EDTA | 10 mM: 36% inhibition, 1 mM: slight activation | Euonymus alatus | |
iodoacetamide | 1 mM, 28% inhibition | Euonymus alatus | |
iodoacetate | 1 mM, 16% inhibition | Euonymus alatus | |
KCl | 1 or 10 mM, 31% inhibition | Euonymus alatus | |
Mg2+ | 10 mM, 20% inhibition | Euonymus alatus | |
Mn2+ | 10 mM, 37% inhibition | Euonymus alatus | |
N-ethylmaleimide | 1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity | Euonymus alatus | |
p-chloromercuribenzoate | 1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity | Euonymus alatus | |
Phenylmercuriacetate | 1 mM, 96% inhibition | Euonymus alatus | |
Zn2+ | 1 mM, 89% inhibition | Euonymus alatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
UDP-glucose | pH 7.5, 30°C, cosubstrate kaempferol | Euonymus alatus | |
0.0111 | - |
kaempferol | pH 7.5, 30°C | Euonymus alatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for Mg2+ | Euonymus alatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Euonymus alatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Euonymus alatus | glycosylation of flavonols | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euonymus alatus | - |
f. Ciliato-dentatus | - |
Purification (Comment) | Organism |
---|---|
139.9fold, partial | Euonymus alatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Euonymus alatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.76 | - |
pH 7.5, 30°C, kaempferol as substrate | Euonymus alatus |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 18% loss of activity, 8 days: 64% loss of activity | Euonymus alatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glycosylation of flavonols | Euonymus alatus | ? | - |
? | |
additional information | no or very poor substrates: fisetin, kaempferol 5,7,4-trimethylether, rhamnetin, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, taxifolin, dihydrokaempferol, ADP-D-glucose, GDP-D-glucose | Euonymus alatus | ? | - |
? | |
UDP-alpha-D-glucose + kaempferol | good substrate | Euonymus alatus | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | 29% of the activity with kaempferol as substrate | Euonymus alatus | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + 3,5,7-trihydroxyflavanone | pinobanksin, 55% of the activity with kaempferol as substrate | Euonymus alatus | UDP + 3,5,7-trihydroxyflavanone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + 7,4'-dihydroxyflavonol | 53% of the activity with kaempferol as substrate | Euonymus alatus | UDP + 7,4'-dihydroxyflavonol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavonol | - |
Euonymus alatus | UDP + a flavonol 7-O-beta-D-glucoside | F7GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some other flavonols, highly specific for UDP-D-glucose as glucosyl donor | ? | |
UDP-glucose + euonymin | 16% of the activity with kaempferol as substrate | Euonymus alatus | UDP + euonymin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + isoquercitrin | 13% of the activity with kaempferol as substrate | Euonymus alatus | UDP + isoquercitrin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + isorhamnetin | 128% of the activity with kaempferol as substrate | Euonymus alatus | UDP + isorhamnetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferide | 104% of the activity with kaempferol as substrate | Euonymus alatus | UDP + kaempferide 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol 3,4'-di-O-glucoside | 12% of the activity with kaempferol as substrate | Euonymus alatus | UDP + kaempferol 3,7,4'-tri-O-glucoside | - |
? | |
UDP-glucose + kaempferol 3,4'-dimethylether | 31% of the activity with kaempferol as substrate | Euonymus alatus | UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol 3-O-glucoside | astragalin, 20% of the activity with kaempferol as substrate | Euonymus alatus | UDP + kaempferol 3,7-O-diglucoside | - |
? | |
UDP-glucose + myricetin | 36% of the activity with kaempferol as substrate | Euonymus alatus | UDP + myricetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + quercetin 5-methylether | 13% of the activity with kaempferol as substrate | Euonymus alatus | UDP + quercetin 5-methylether 7-O-beta-D-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, SDS-PAGE | Euonymus alatus |
Synonyms | Comment | Organism |
---|---|---|
F7GT | - |
Euonymus alatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Euonymus alatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Tris-HCl buffer | Euonymus alatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Euonymus alatus | chromatofocusing | - |
4.5 |