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Literature summary for 2.4.1.155 extracted from

  • Dong, Z.; Zuber, C.; Pierce, M.; Stanley, P.; Roth, J.
    Reduction in Golgi apparatus dimension in the absence of a residential protein, N-acetylglucosaminyltransferase V (2014), Histochem. Cell Biol., 141, 153-164.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.155

Cloned(Commentary)

Cloned (Comment) Organism
gene MGAT5, overexpression in CHO cells and Lec4 cells from Cricetulus griseus Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information generation of cell line Lec4A lacking N-acetylglucosaminyltransferase V activity and protein in the Golgi apparatus Cricetulus griseus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Cricetulus griseus 5794
-
Golgi apparatus
-
 Rattus norvegicus 5794
-

Organism

Organism UniProt Comment Textmining
Cricetulus griseus P97259 gene MGAT5
-
Rattus norvegicus Q08834 gene MGAT5
-

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
 Cricetulus griseus
-
CHO-Lec4 cell
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + octyl 6-O-[2-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-alpha-D-mannopyranosyl]-beta-D-glucopyranoside a synthetic trisaccharide acceptor Cricetulus griseus UDP + ?
-
 ?
UDP-N-acetyl-D-glucosamine + octyl 6-O-[2-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-alpha-D-mannopyranosyl]-beta-D-glucopyranoside a synthetic trisaccharide acceptor Rattus norvegicus UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
Glc-NAcT-V
-
 Cricetulus griseus
Glc-NAcT-V
-
 Rattus norvegicus
GlcNAcT-V
-
 Cricetulus griseus
GlcNAcT-V
-
 Rattus norvegicus
Mgat5
-
 Cricetulus griseus
Mgat5
-
 Rattus norvegicus
N-acetylglucosaminyltransferase V
-
 Cricetulus griseus
N-acetylglucosaminyltransferase V
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cricetulus griseus
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Cricetulus griseus
6.5
-
 assay at Rattus norvegicus

General Information

General Information Comment Organism
malfunction absense of the enzyme is due to a base insertion at nucleotide 822 of the Magt5 gene that shifts the open reading frame. A 155 amino acid truncated GlcNAcT-V (instead of a full length 740 amino acid enzyme) may be synthesized, which consists of the cytosolic and transmembrane domains and a short piece of the stem region, the truncated enzyme is degenerated. Lack of the enzyme protein causes a reduction in Golgi volume density in Lec4A cells, this can be reversed by stable transfection of Lec4A cells with a cDNA encoding Mgat5. No effect on Golgi volume density is observed in CHO Lec1 cells that contain enzymatically active GlcNAcT-V, but cannot synthesize beta1,6-branched glycans due to an inactive GlcNAcT-I in their Golgi apparatus. The structure of the Golgi apparatus in cells stably transfected and therefore overexpressing different glycosyltransferases appears normal Cricetulus griseus
malfunction absense of the enzyme is due to a base insertion at nucleotide 822 of the Magt5 gene that shifts the open reading frame. A 155 amino acid truncated GlcNAcT-V (instead of a full length 740 amino acid enzyme) may be synthesized, which consists of the cytosolic and transmembrane domains and a short piece of the stem region, the truncated enzyme is degenerated. Lack of the enzyme protein causes a reduction in Golgi volume density in Lec4A cells, this can be reversed by stable transfection of Lec4A cells with a cDNA encoding Mgat5. No effect on Golgi volume density is observed in CHO Lec1 cells that contain enzymatically active GlcNAcT-V, but cannot synthesize beta1,6-branched glycans due to an inactive GlcNAcT-I in their Golgi apparatus. The structure of the Golgi apparatus in cells stably transfected and therefore overexpressing different glycosyltransferases appears normal Rattus norvegicus