Cloned (Comment) | Organism |
---|---|
GPase gene, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, expression in Escherichia coli strain DH5alpha | Artemia sinica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | an allosteric enzyme existing as two interconvertible forms: phosphorylase a (active type) and phosphorylase b (inactive type) | Artemia sinica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Artemia sinica | 5737 | - |
membrane | - |
Artemia sinica | 16020 | - |
additional information | prediction of protein subcellular localization of the GPase indicates that 52.3% is located in the cytoplasm, 21.7% in the nucleus, 17.4% in the mitochondria and 4.3% in the cytoskeleton and plasma membrane | Artemia sinica | - |
- |
nucleus | - |
Artemia sinica | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92500 | - |
2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation | Artemia sinica |
98190 | - |
2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation | Artemia sinica |
190000 | - |
about | Artemia sinica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate | Artemia sinica | - |
[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemia sinica | I3QKR5 | collected from a salt lake, YunCheng, Shanxi Province, China | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Artemia sinica | - |
cyst | - |
Artemia sinica | - |
additional information | wide distribution of the GPase gene during different developmental stages, expression analysis, overview | Artemia sinica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Artemia sinica | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation | Artemia sinica |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4 glucan phosphorylase | UniProt | Artemia sinica |
GPase | - |
Artemia sinica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Artemia sinica | sequence calcualtion | - |
5.67 |
Organism | Comment | Expression |
---|---|---|
Artemia sinica | expression patterns of the glycogen phosphorylase gene during development and in response to temperature stress, overview. Expression is downregulated at temperatures ranging from 20°C to 25°C and is subsequently upregulated in the range 5-15°C | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | glycogen phosphorylase is a key enzyme involved in intracellular glycogen catabolism, catalyzing the first step in glycogen degradation. In the diapause, GPase catalyzes glycogen into the closely related molecule, sorbitol. The GPase gene expression is stress-related and might play an important role in Artemia development and metabolism | Artemia sinica |