Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | activation | Lethenteron camtschaticum | |
Na2SO4 | 500 mM, 40% activation of phosphorylase b in the presence of AMP | Lethenteron camtschaticum | |
phosphate | activation | Lethenteron camtschaticum | |
phosphate | in the presence of ATP-Mg | Lethenteron camtschaticum | |
phosphate | converts phosphorylase b into phosphorylase a | Lethenteron camtschaticum | |
Phosphorylase kinase | activation | Lethenteron camtschaticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Lethenteron camtschaticum | |
additional information | - |
additional information | comparison of muscle phosphorylases of various animals | Lethenteron camtschaticum | |
0.1 | - |
AMP | phosphorylase b | Lethenteron camtschaticum | |
3.5 | - |
alpha-D-glucose 1-phosphate | phosphorylase a | Lethenteron camtschaticum | |
15 | - |
alpha-D-glucose 1-phosphate | phosphorylase b, pH 6.3 | Lethenteron camtschaticum | |
22 | - |
alpha-D-glucose 1-phosphate | phosphorylase b, pH 6.8 | Lethenteron camtschaticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
2 * 94000, SDS-PAGE | Lethenteron camtschaticum |
170000 | - |
native PAGE | Lethenteron camtschaticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lethenteron camtschaticum | - |
lamprey | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | activation by rabbit muscle calcium-activated phosphorylase kinase in the presence of ATP and Mg2+ | Lethenteron camtschaticum |
side-chain modification | phosphorylation converts inactive phosphorylase b into active phosphorylase a | Lethenteron camtschaticum |
Purification (Comment) | Organism |
---|---|
pH 5.4, ammonium sulfate, DEAE-cellulose, 5'-AMP-Sepharose | Lethenteron camtschaticum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | tissue distribution | Lethenteron camtschaticum | - |
muscle | skeletal muscle | Lethenteron camtschaticum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60 | - |
phosphorylase a, in the absence of AMP | Lethenteron camtschaticum |
75 | - |
phosphorylase a, in the presence of 1 mM AMP | Lethenteron camtschaticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Lethenteron camtschaticum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 94000, SDS-PAGE | Lethenteron camtschaticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Lethenteron camtschaticum |