General Stability | Organism |
---|---|
dilution destabilizes | Quercus robur |
glycerol, 10%, stabilizes | Quercus robur |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | above 50 mM | Quercus robur | |
dithioerythritol | above 20 mM | Quercus robur | |
additional information | no inhibition by metal ions | Quercus robur |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | normal Michaelis-Menten kinetics | Quercus robur | |
additional information | - |
additional information | substrate saturation not reached at 20 mM | Quercus robur |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Quercus robur |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | - |
gel filtration | Quercus robur |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose | Quercus robur | first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor | 1,6-di-O-galloyl-beta-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Quercus robur | - |
oak | - |
Purification (Comment) | Organism |
---|---|
partial purification with ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration | Quercus robur |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | 2-3 months old | Quercus robur | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0743 | - |
- |
Quercus robur |
Storage Stability | Organism |
---|---|
-20°C, stable in the presence of 10% glycerol | Quercus robur |
2-4°C, t1/2: 2 days, dilute preparation | Quercus robur |
2-4°C, t1/2: 7 days, concentrated preparation | Quercus robur |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose | - |
Quercus robur | 1,6-di-O-galloyl-beta-D-glucose + D-glucose | - |
? | |
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose | first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor | Quercus robur | 1,6-di-O-galloyl-beta-D-glucose + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
25% residual activity at 0°C | Quercus robur |
30 | - |
- |
Quercus robur |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
highest stability at pH 4.5-6.5 | Quercus robur |
55 | 60 | denaturation | Quercus robur |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Quercus robur |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | about half-maximal activity at pH 5.5 and pH 7.5 | Quercus robur |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | highest stability at 30°C | Quercus robur |