Cloned (Comment) | Organism |
---|---|
expression in HeLa cell | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
modelling predicts that ATP binds within the active site cleft arranged with the triphosphate group in close proximity to arginine 127 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K100Q | mutation decreases the potency of ATP as an inhibitor of NAT1. The Hill coefficient increases twofold | Homo sapiens |
K100R | mutation decreases the potency of ATP as an inhibitor of NAT1. The Hill coefficient increases threefold | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | non-competitive inhibitor with respect to the acetyl acceptor, competitive inhibitor with respect to acetyl-coenzyme A. There is no effect by presence or absence of Mg2+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
4-Aminobenzoate | presence of 1 mM ATP, 37°C, pH not specified in the publication | Homo sapiens | |
0.053 | - |
4-Aminobenzoate | 37°C, pH not specified in the publication | Homo sapiens | |
0.242 | - |
acetyl-CoA | 37°C, pH not specified in the publication | Homo sapiens | |
0.651 | - |
acetyl-CoA | presence of 1 mM ATP, 37°C, pH not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P18440 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
acetylation | lysine 100 is a site of posttranslational modification by acetylation | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 4-aminobenzoate | - |
Homo sapiens | CoA + N-acetyl-4-aminobenzoate | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.7 | - |
wild-type, 37°C, pH not specified in the publication | Homo sapiens | ATP | |
3.8 | - |
mutant K100R, 37°C, pH not specified in the publication | Homo sapiens | ATP | |
3.9 | - |
mutant K100Q, 37°C, pH not specified in the publication | Homo sapiens | ATP |