Literature summary for 2.3.1.269 extracted from

Wiktor, M.; Weichert, D.; Howe, N.; Huang, C.Y.; Olieric, V.; Boland, C.; Bailey, J.; Vogeley, L.; Stansfeld, P.J.; Buddelmeijer, N.; Wang, M.; Caffrey, M.
Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis (2017), Nat. Commun., 8, 15952 .

Search for more literature for 2.3.1.269

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C41(DE3) cells	Escherichia coli
expressed in Escherichia coli C41(DE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate	Pseudomonas aeruginosa
using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
C387A	inactive	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-
membrane	-	Pseudomonas aeruginosa	16020	-

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P23930	-	-
Pseudomonas aeruginosa	Q9ZI86	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Escherichia coli
Ni-NTA column chromatography and Superdex 200 gel filtration	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine	-	Escherichia coli	1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine	-	?
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine	-	Pseudomonas aeruginosa	1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine	-	?

Synonyms

Synonyms	Comment	Organism
lnt	-	Escherichia coli
lnt	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)