Cloned (Comment) | Organism |
---|---|
gene MG61, DNA and amino acid sequence determination and analysis, sequence comparisons | Homo sapiens |
gene mom-1, DNA and amino acid sequence determination and analysis, sequence comparisons | Caenorhabditis elegans |
gene Porc, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in HEK-293 cells with or without co-expression of Wg | Drosophila melanogaster |
gene porc, expression of the enzyme tagged with three HA epitopes at amino acid 28. Capped porc, porc3HA28, DELTA34, DELTA66, and DELTA110 RNA are prepared by in vitro transcription and RNA is injected into Drosophila embryos | Drosophila melanogaster |
gene Xporc, DNA and amino acid sequence determination and analysis, sequence comparisons | Xenopus laevis |
single gene Mporc, variants by alternative splicing, DNA and amino acid sequence determination and analysis, sequence comparisons, co-expression in COS-1 cells of HA-tagged Mporc and either Myc-tagged Wnt or partial laminin beta chain, recombinant expression in HEK-293 cells with or without co-expression of Drosophila melanogaster Wnt variant Wg | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview | Drosophila melanogaster |
additional information | rescue of Drosophila melanogaster porc embryos by Mporc RNA injection | Mus musculus |
additional information | rescue of Drosophila porc embryos by Mus musculus Mporc RNA injection | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | a multispanning transmembrane protein | Drosophila melanogaster | 5789 | - |
endoplasmic reticulum membrane | the enzyme has multiple transmembrane domains | Homo sapiens | 5789 | - |
endoplasmic reticulum membrane | the enzyme has multiple transmembrane domains | Mus musculus | 5789 | - |
endoplasmic reticulum membrane | the enzyme has multiple transmembrane domains | Xenopus laevis | 5789 | - |
endoplasmic reticulum membrane | the enzyme has multiple transmembrane domains | Caenorhabditis elegans | 5789 | - |
endoplasmic reticulum membrane | the enzyme has multiple transmembrane domains, Drosophila Porc is localized at the endoplasmic reticulum when expressed in Drosophila cells as an epitope-tagged protein | Drosophila melanogaster | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine | Drosophila melanogaster | - |
CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine | Drosophila melanogaster | - |
CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine | Drosophila melanogaster | the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg | CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | Homo sapiens | - |
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | Mus musculus | - |
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | Drosophila melanogaster | - |
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | Xenopus laevis | - |
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | Caenorhabditis elegans | - |
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
additional information | Drosophila melanogaster | enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | Q22329 | gene mom-1; gene mom-1 | - |
Drosophila melanogaster | - |
gene porcupine, i.e. porc | - |
Drosophila melanogaster | Q9VWV9 | gene Porc or CG6205 | - |
Homo sapiens | Q9H237 | gene porc | - |
Mus musculus | Q9JJJ7 | single gene and alternative splicing resulting in several variants | - |
Xenopus laevis | Q9I935 | gene Xporc | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Drosophila melanogaster | - |
embryo | - |
Drosophila melanogaster | - |
embryo | - |
Mus musculus | - |
fibroblast | - |
Mus musculus | - |
HEK-293 cell | - |
Homo sapiens | - |
additional information | Mporc mRNA is differentially expressed during embryogenesis and in various adult tissues, demonstrating that the alternative splicing is regulated to synthesize the specific types of Mporc, pattern of expression of Mporc at fetal and adult stages of development, overview | Mus musculus | - |
oocyte | - |
Xenopus laevis | - |
S2 cell | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine | - |
Drosophila melanogaster | CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine | Wnt substrate from Drosophila melanogaster | Mus musculus | CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine | - |
Drosophila melanogaster | CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine | - |
Drosophila melanogaster | CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine | the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg | Drosophila melanogaster | CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine | the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg. The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg | Drosophila melanogaster | CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | - |
Homo sapiens | CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | - |
Mus musculus | CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | - |
Drosophila melanogaster | CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | - |
Xenopus laevis | CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | - |
Caenorhabditis elegans | CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
additional information | enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity | Drosophila melanogaster | ? | - |
? | |
additional information | recombinant c-Myc-tagged substrate Wg mutants T51A, S105A, S110A, and T416A | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CG6205 | - |
Drosophila melanogaster |
MG61 | - |
Homo sapiens |
Mom-1 | - |
Caenorhabditis elegans |
Mporc | - |
Mus musculus |
PORC | - |
Drosophila melanogaster |
PORC | - |
Homo sapiens |
PORCN | - |
Homo sapiens |
PORCN | - |
Caenorhabditis elegans |
PPN | - |
Homo sapiens |
Xporc | - |
Xenopus laevis |
General Information | Comment | Organism |
---|---|---|
evolution | Drosophila Porc has an extra hydrophilic N-terminal sequence, which is not found in other Porc enzymes | Drosophila melanogaster |
additional information | all Mporc types can substitute for Drosophila Porc, as they are able to rescue the phenotypes of Drosophila porc embryos, the enzyme modifies the processing of Drosophila Wg protein expressed in mammalian cells | Mus musculus |
additional information | all Mus musculus Mporc types can substitute for Drosophila Porc, as they are able to rescue the phenotypes of Drosophila porc embryos | Drosophila melanogaster |
additional information | model for the N-glycosylation of Wnt family, overview | Drosophila melanogaster |
physiological function | ectopic expression of porc stimulates the N-glycosylation of both endogenously and exogenously expressed protein Wingless (Wg). The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg, which is highly conserved in the Wnt family and stimulates the N-glycosylation at surrounding sites. The enzyme is also necessary for the processing of Drosophila Wnt-3/5 in both embryos and cultured cells. The enzyme binds the N-terminal specific domain of the Wnt family via its C-terminnus and stimulates its posttranslational N-glycosylation by anchoring them at the endoplasmic reticulum membrane possibly through acylation, overview | Drosophila melanogaster |
physiological function | Mporc binds Wnt proteins and modifies their processing, the enzyme may function as a chaperone-like molecule for Wnt, overview | Mus musculus |
physiological function | the enzyme is necessary for processing Wingless (Wg), a Drosophila Wnt (Wnt) family member. The enzyme modifies the processing of substrate Wg expressed in mammalian cells | Drosophila melanogaster |