Application | Comment | Organism |
---|---|---|
agriculture | the enzyme may serve as a tool for the metabolic engineering of flavonoid biosynthesis leading to the accumulation of cinerarin in flowers, which results in the coloration of blue flowers | Pericallis cruenta |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 under the control of T5 promoter as an in-frame N-terminal fusion with a His6 tag | Pericallis cruenta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | 0.1 mM, 63% inhibition | Pericallis cruenta | |
CdCl2 | 0.1 mM, 48% loss of activity | Pericallis cruenta | |
CoA | 0.1 mM, 67% inhibition | Pericallis cruenta | |
CuCl2 | 0.1 mM, complete inhibition | Pericallis cruenta | |
HgCl2 | 0.1 mM, complete inhibition | Pericallis cruenta | |
MgCl2 | 0.1 mM, 52% inhibition | Pericallis cruenta | |
additional information | 0.1 mM EDTA or 0.1 mM CaCl2, CoCl2, FeCl2, MnCl2, NiCl2 or SnCl2 have negligible effects on the enzyme activity | Pericallis cruenta | |
NEM | 5 mM, complete inhibition | Pericallis cruenta | |
ZnCl2 | 0.1 mM, 66% inhibition | Pericallis cruenta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
pelargonidin 3-O-beta-D-glucoside | - |
Pericallis cruenta | |
0.061 | - |
malonyl-CoA | - |
Pericallis cruenta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + delphinidin 3-O-beta-D-glucoside | Pericallis cruenta | because the reaction product delphinidin 3-O-6'-malonylglucoside, is a part of cinerarin biosynthesis, it is highly likely that Sc3MaT is involved in the biosynthesis of cinerarin | CoA + delphinidin 3-O-(6-malonyl-beta-D-glucoside) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pericallis cruenta | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Pericallis cruenta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + cyanidin 3-O-beta-D-glucoside | 110% of the activity with pelargonidin 3-O-glucoside | Pericallis cruenta | CoA + cyanidin 3-O-(6-O-malonyl-beta-D-glucoside) | - |
? | |
malonyl-CoA + delphinidin 3-O-beta-D-glucoside | because the reaction product delphinidin 3-O-6'-malonylglucoside, is a part of cinerarin biosynthesis, it is highly likely that Sc3MaT is involved in the biosynthesis of cinerarin | Pericallis cruenta | CoA + delphinidin 3-O-(6-malonyl-beta-D-glucoside) | - |
? | |
malonyl-CoA + delphinidin 3-O-beta-D-glucoside | 153% of the activity with pelargonidin 3-O-glucoside | Pericallis cruenta | CoA + delphinidin 3-O-(6-O-malonyl-beta-D-glucoside) | - |
? | |
malonyl-CoA + pelargonidin 3-O-beta-D-glucoside | - |
Pericallis cruenta | CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside) | - |
? | |
methylmalonyl-CoA + pelargonidin 3-O-beta-D-glucoside | 15% of the activity with methylmalonyl-CoA | Pericallis cruenta | CoA + pelargonidin 3-O-(6-O-methylmalonyl-beta-D-glucoside) | - |
? | |
succinyl-CoA + pelargonidin 3-O-beta-D-glucoside | 20% of the activity with methylmalonyl-CoA | Pericallis cruenta | CoA + pelargonidin 3-O-(6-O-succinyl-beta-D-glucoside) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3MaT | - |
Pericallis cruenta |
anthocyanidin 3-glucoside malonyltransferase | - |
Pericallis cruenta |
anthocyanin 3-glucoside malonyltransferase | - |
Pericallis cruenta |
Dv3MaT | - |
Pericallis cruenta |
malonyl-CoA:anthocyanin 3-O-glucoside-6'-O-malonyltransferase | - |
Pericallis cruenta |
malonyl-coenzyme A:anthocyanidin 3-O-glucoside 6''-O-malonyltransferase | - |
Pericallis cruenta |
malonyl-coenzyme A:anthocyanidin-3-O-beta-D-glucoside 6''-O-malonyltransferase | - |
Pericallis cruenta |
malonyltransferase, anthocyanidin 3-glucoside | - |
Pericallis cruenta |
Sc3MaT | - |
Pericallis cruenta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.0, 20 min, stable below | Pericallis cruenta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.8 | - |
malonyl-CoA | - |
Pericallis cruenta | |
8.8 | - |
pelargonidin 3-O-beta-D-glucoside | - |
Pericallis cruenta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pericallis cruenta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | pH 7.0, 20 min, stable | Pericallis cruenta |