Cloned (Comment) | Organism |
---|---|
ATAT1, DNA and amino acid sequence determination and analysis, stable functional expression of EGFP-ATAT1 and mutant ATAT1D157N in MDA-MB-231 cells and in HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D157N | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
additional information | overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + cortactin | Homo sapiens | ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells | CoA + N-acetyl-cortactin | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | Homo sapiens | acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MDA-MB-231 cell | invasive breast cancer cells | Homo sapiens | - |
additional information | ATAT1 colocalizes with cortactin at the adherent surface of the cells, association of ATAT1 and cortactin at invadopodia, subcellular localization study, overview | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + cortactin | - |
Homo sapiens | CoA + N-acetyl-cortactin | - |
? | |
acetyl-CoA + cortactin | ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells | Homo sapiens | CoA + N-acetyl-cortactin | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | - |
Homo sapiens | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen | Homo sapiens | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-tubulin N-acetyltransferase 1 | - |
Homo sapiens |
ATAT1 | - |
Homo sapiens |
ATAT1 acetylase | - |
Homo sapiens |
ATAT1 tubulin acetyltransferase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin. Random invasive migration of cells in 3D collagen I is inhibited by 40-50% upon MT1-MMP or ATAT1 knockdown | Homo sapiens |
metabolism | a balance of acetylation and deaceylation by ATAT1/HDAC6, histone deacetylase 6, enzymes with opposite activities regulates the migratory and invasive capacities of breast tumor cells | Homo sapiens |
physiological function | dynamics and distribution of MT1-MMP-positive endosomes require regulation of acetylation levels, ATAT1 tubulin acetyltransferase binds and regulates cortactin acetylation levels. Acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen. ATAT1 colocalizes with cortactin at the adherent surface of the cells and it is required for 2D migration and invasive migration of MDA-MB-231 cells in collagen matrix | Homo sapiens |