Protein Variants | Comment | Organism |
---|---|---|
K219Q | no residual activity, no binding of FAD | Nicotiana tabacum |
K255F | strong resistance to Londax, Cadre and N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | Nicotiana tabacum |
K255Q | strong resistance to Londax, Cadre and N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | Nicotiana tabacum |
K299Q | no significant effects | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | - |
Nicotiana tabacum | |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | - |
Nicotiana tabacum | |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | ratio 1:1 | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.06 | - |
pyruvate | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
26.23 | - |
pyruvate | mutant K299Q pH 7.5, 37°C | Nicotiana tabacum | |
109.4 | - |
pyruvate | mutant K255F, pH 7.5, 37°C | Nicotiana tabacum | |
115.5 | - |
pyruvate | mutant K255Q, pH 7.5, 37°C | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.02 | - |
mutant K255F, pH 7.5, 37°C | Nicotiana tabacum |
1.87 | - |
wild-type, pH 7.5, 37°C | Nicotiana tabacum |
2.17 | - |
mutant K255Q, pH 7.5, 37°C | Nicotiana tabacum |
4.15 | - |
mutant K299Q pH 7.5, 37°C | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000823 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.00000902 | - |
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide | mutant K299Q pH 7.5, 37°C | Nicotiana tabacum | |
0.00109 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | mutant K299Q pH 7.5, 37°C | Nicotiana tabacum | |
0.00122 | - |
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one | wild-type, pH 7.5, 37°C | Nicotiana tabacum | |
0.00301 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | mutant K299Q pH 7.5, 37°C | Nicotiana tabacum | |
0.00527 | - |
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide | wild-type, pH 7.5, 37°C | Nicotiana tabacum |